Biodiversity decline undermines the conditions for life on Earth resulting in calls for transformative governance of biodiversity. Under the Convention on Biological Diversity, national biodiversity strategies provide the primary mechanism through which governments demonstrate their conservation efforts. With many countries due to develop new strategies under the Post-2020 Global Biodiversity Framework, it is timely to assess existing ones to identify policy design elements that could be ‘ratcheted up’ to meet the transformative agenda. This article analyzes andc ompares the policy designs of national biodiversity strategies in Australia, France and Sweden. We cover problem framing, policy goals, targeted groups, implementing agents, and policy instruments, to draw lessons on how national strategies can be designed to further support transformation of biodiversity governance. We identify elements in these strategies that can be used to inspire future ones: a negotiated framing of biodiversity and participatory processes in France, nested and integrated goals, targets and measures in Sweden, and an engagement with indigenous knowledge in Australia. However, to bring about transformative change, the analysis also shows the need for novel and fundamental re-designs to successfully target indirect drivers of biodiversity loss, shift power relations, and make biodiversity conservation a priority rather than an option.