To Örebro University

oru.seÖrebro University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Production of anti-viral vaccines using probiotic bacteria
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-9713-2365
Örebro University, School of Medical Sciences. Örebro University.ORCID iD: 0000-0001-6819-7960
Karolinska Institutet, Stockholm, Sweden.
University of Cape Town, Cape Town, South Africa.
Show others and affiliations
2022 (English)In: 19th Smögen Symposium on Virology: Abstracts: Viral Immunology and Vaccines II and III, Virus- och Pandemifonden – Swedish Society for Virology , 2022, p. 16-16Conference paper, Oral presentation with published abstract (Refereed)
Abstract [en]

The mucosal surfaces throughout the body are constantly exposed to microorganisms. The mucosal surfaces accommodate a large part of the body’s immune system. For effective vaccination, it is believed that direct immunization on mucosal surfaces will be more effective than the more conventional systemic immunization. Certain probiotic bacteria provide significant adjuvant effects that may be utilized for the desired immune response. Virus-like particles (VLPs) are complexes of viral proteins that without being infectious are efficient in mimicking the natural viral structures. While presenting the patterns of viral antigens, the VLPs have been shown to efficiently interact with dendritic cells and be effective in triggering the B and T-cell immunity. HIV-1 Gag is one of the most highly conserved structural antigens and contains several immunodominant T- and B-cell epitopes. Mosaic Gag protein matches 74% of 9-amino-acid potential epitopes in global Gag sequences thus maximizing the coverage of potential T-cell epitopes for a viral population.The current study aimed to develop probiotic strains of Lactobacillus plantarum NC8 and E. coli Nissle 1917 that produced recombinant mosaic HIV-1 Gag protein as VLPs.For the transient expression of the mosaic HIV-1 Gag protein (GagM), the gene-carrying expression vectors were transformed into the L. plantarum and in probiotic E.coli Nissle 1917. Protein expression of the GagM was shown to lead to the formation of the VLPs of the recombinant HIV-1 Gag proteins. This was confirmed through immunoblotting and transmission electron microscopy (TEM).Our study shows that probiotic lactobacteria can be developed to express HIV-1 Gag VLPs, which may be used for VLP production and potentially for vaccine delivery. Further evaluation of the concept is merited.

Place, publisher, year, edition, pages
Virus- och Pandemifonden – Swedish Society for Virology , 2022. p. 16-16
National Category
Immunology
Research subject
Immunology
Identifiers
URN: urn:nbn:se:oru:diva-101080OAI: oai:DiVA.org:oru-101080DiVA, id: diva2:1692986
Conference
19th Smögen Symposium on Virology, Smögen, August 25-27, 2022
Projects
Developvaccines@oru
Funder
Knowledge FoundationAvailable from: 2022-09-05 Created: 2022-09-05 Last updated: 2022-09-06Bibliographically approved

Open Access in DiVA

Fulltext(230 kB)58 downloads
File information
File name FULLTEXT02.pdfFile size 230 kBChecksum SHA-512
0e1075df484155d64d7214d28cca823aac04d8f207c86a1f7c7eba6eb50ce6c6070e30c605b94cf941cd3883129ab48685ab0efcfd31b1a6292c38c1539846d7
Type fulltextMimetype application/pdf

Authority records

Scherbak, NikolaiNinyio, NathanielAndersson, Sören

Search in DiVA

By author/editor
Scherbak, NikolaiNinyio, NathanielAndersson, Sören
By organisation
School of Science and TechnologySchool of Medical Sciences
Immunology

Search outside of DiVA

GoogleGoogle Scholar
Total: 58 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 188 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf