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Dynamics of the Lipidome in a Colon Simulator
Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland.
Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland.
Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland.
Global Health and Nutrition Sciences, International Flavors & Fragrances, 02460 Kantvik, Finland.
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2023 (English)In: Metabolites, ISSN 2218-1989, E-ISSN 2218-1989, Vol. 13, no 3, article id 355Article in journal (Refereed) Published
Abstract [en]

Current evidence suggests that gut microbiome-derived lipids play a crucial role in the regulation of host lipid metabolism. However, not much is known about the dynamics of gut microbial lipids within the distinct gut biogeographic. Here we applied targeted and untargeted lipidomics to in vitro-derived feces. Simulated intestinal chyme was collected from in vitro gut vessels (V1-V4), representing proximal to distal parts of the colon after 24 and 48 h with/without polydextrose treatment. In total, 44 simulated chyme samples were collected from the in vitro colon simulator. Factor analysis showed that vessel and time had the strongest impact on the simulated intestinal chyme lipid profiles. We found that levels of phosphatidylcholines, sphingomyelins, triacylglycerols, and endocannabinoids were altered in at least one vessel (V1-V4) during simulation. We also found that concentrations of triacylglycerols, diacylglycerols, and endocannabinoids changed with time (24 vs. 48 h of simulation). Together, we found that the simulated intestinal chyme revealed a wide range of lipids that remained altered in different compartments of the human colon model over time.

Place, publisher, year, edition, pages
MDPI, 2023. Vol. 13, no 3, article id 355
Keywords [en]
Gut microbiome, in vitro colon simulator, lipidomics, metabolomics
National Category
Bioinformatics (Computational Biology)
Identifiers
URN: urn:nbn:se:oru:diva-105217DOI: 10.3390/metabo13030355ISI: 000958186800001PubMedID: 36984795Scopus ID: 2-s2.0-85151516411OAI: oai:DiVA.org:oru-105217DiVA, id: diva2:1747548
Available from: 2023-03-30 Created: 2023-03-30 Last updated: 2023-04-17Bibliographically approved

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Hyötyläinen, TuuliaOresic, Matej

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