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Elution of antitransglutaminase antibodies from duodenal biopsies: a novel approach in the diagnosis of celiac disease
Department of Laboratory Medicine/Microbiology, Örebro University Hospital, Örebro, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Division of Gastroenterology, Department of Medicine, Örebro University Hospital, Örebro, Sweden.
Department of Laboratory Medicine/Pathology, Örebro University Hospital, Örebro, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Department of Laboratory Medicine/Microbiology, Örebro University Hospital, Örebro, Sweden.
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2012 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 120, no 8, p. 666-674Article in journal (Refereed) Published
Abstract [en]

Celiac disease (CeD) is a disease more prevalent and multisymptomatic than was earlier recognized. Whereas prompt initiation of gluten-free diet (GFD) is beneficial in relieving the symptoms, an accurate CeD diagnosis is necessary also to avoid years of restricted diet on uncertain grounds. We propose a new diagnostic method, based on elution of deposited antibodies against transglutaminase (anti-tTG) from duodenal biopsies in patients with symptoms and screening serology analyses suggestive of CeD. The eluates were analyzed in a Phadia 250 fluoroimmunoassay, demonstrating elevated concentrations of anti-tTG in CeD patients, corresponding to serology and histopathology findings. In one case histology was inconclusive, displaying only unspecific inflammation, but eluted anti-tTG was positive. This patient has clinically improved following GFD. We conclude that our novel method represents a new tool in the diagnostic work up in CeD. The detection of deposited anti-tTG at the site of inflammation appears to provide a high sensitivity and specificity using a technique that is quick, simple and reliable. Further studies are needed for optimization and elucidation of suitable applications for this elution method.

Place, publisher, year, edition, pages
Hoboken, USA: Wiley-Blackwell, 2012. Vol. 120, no 8, p. 666-674
Keywords [en]
Celiac disease, antitransglutaminase antibodies, elution, borderline cases
National Category
Medical and Health Sciences Immunology
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-24221DOI: 10.1111/j.1600-0463.2012.02884.xISI: 000306310500009PubMedID: 22779690Scopus ID: 2-s2.0-84863836943OAI: oai:DiVA.org:oru-24221DiVA, id: diva2:542839
Available from: 2012-08-03 Created: 2012-08-03 Last updated: 2017-12-07Bibliographically approved

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Tysk, CurtHultgren, Olof

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