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Effects of gadolinium oxide nanoparticles on the oxidative burst from human neutrophil granulocytes
Division of Molecular Surface Physics and Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden .
Division of Molecular Surface Physics and Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden; Division of Clinical Medicine, Department of Biomedicine, Örebro University, Örebro, Sweden.
Division of Molecular Surface Physics and Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Division of Clinical Medicine, Department of Biomedicine, School of Health and Medical Sciences, Örebro University, Örebro, Sweden.
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2012 (English)In: Nanotechnology, ISSN 0957-4484, E-ISSN 1361-6528, Vol. 23, no 27, article id 275101Article in journal (Refereed) Published
Abstract [en]

We have previously shown that gadolinium oxide (Gd2O3) nanoparticles are promising candidates to be used as contrast agents in magnetic resonance (MR) imaging applications. In this study, these nanoparticles were investigated in a cellular system, as possible probes for visualization and targeting intended for bioimaging applications. We evaluated the impact of the presence of Gd2O3 nanoparticles on the production of reactive oxygen species (ROS) from human neutrophils, by means of luminol-dependent chemiluminescence. Three sets of Gd2O3 nanoparticles were studied, i.e. as synthesized, dialyzed and both PEG-functionalized and dialyzed Gd2O3 nanoparticles. In addition, neutrophil morphology was evaluated by fluorescent staining of the actin cytoskeleton and fluorescence microscopy. We show that surface modification of these nanoparticles with polyethylene glycol (PEG) is essential in order to increase their biocompatibility. We observed that the as synthesized nanoparticles markedly decreased the ROS production from neutrophils challenged with prey (opsonized yeast particles) compared to controls without nanoparticles. After functionalization and dialysis, more moderate inhibitory effects were observed at a corresponding concentration of gadolinium. At lower gadolinium concentration the response was similar to that of the control cells. We suggest that the diethylene glycol (DEG) present in the as synthesized nanoparticle preparation is responsible for the inhibitory effects on the neutrophil oxidative burst. Indeed, in the present study we also show that even a low concentration of DEG, 0.3%, severely inhibits neutrophil function. In summary, the low cellular response upon PEG-functionalized Gd2O3 nanoparticle exposure indicates that these nanoparticles are promising candidates for MR-imaging purposes.

Place, publisher, year, edition, pages
Bristol, United Kingdom: IOP Publishing Ltd. , 2012. Vol. 23, no 27, article id 275101
National Category
Medical and Health Sciences Nano Technology Materials Chemistry
Research subject
Medicine
Identifiers
URN: urn:nbn:se:oru:diva-24621DOI: 10.1088/0957-4484/23/27/275101ISI: 000305802000001PubMedID: 22706406Scopus ID: 2-s2.0-84862659881OAI: oai:DiVA.org:oru-24621DiVA, id: diva2:545835
Funder
VINNOVASwedish Research CouncilSwedish Heart Lung Foundation
Note

Funding Agenicies:

program Innovations for future health, Multifunctional Nanoprobes for Biomedical Visualization

CTS 

Olle Engkvist 

CeNano

Mats Klebergs 

Lars Hiertas Foundation 

Available from: 2012-08-21 Created: 2012-08-21 Last updated: 2018-05-09Bibliographically approved

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Bengtsson, Torbjörn

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