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Multilocus sequence typing and repetitive-sequence-based PCR (DiversiLab) for molecular epidemiological characterization of Propionibacterium acnes isolates of heterogeneous origin
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Department of Urology, Örebro University Hospital, Örebro, Sweden.
Örebro University, School of Medicine, Örebro University, Sweden. Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden.ORCID iD: 0000-0001-5939-2932
Örebro University, School of Health and Medical Sciences. Department of Clinical Microbiology, Umeå University, Umeå, Sweden.
Department of Clinical Microbiology, Umeå University, Umeå, Sweden.
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2012 (English)In: Anaerobe, ISSN 1075-9964, E-ISSN 1095-8274, Vol. 18, no 4, p. 392-399Article in journal (Refereed) Published
Abstract [en]

Propionibacterium acnes is a gram-positive bacillus predominantly found on the skin. Although it is considered an opportunistic pathogen it is also been associated with severe infections. Some specific P. acnes subtypes are hypothesized to be more prone to cause infection than others. Thus, the aim of the present study was to investigate the ability to discriminate between P. acnes isolates of a refined multilocus sequence typing (MLST) method and a genotyping method, DiversiLab, based on repetitive-sequence-PCR technology.

The MLST and DiversiLab analysis were performed on 29 P. acnes isolates of diverse origins; orthopedic implant infections, deep infections following cardiothoracic surgery, skin, and isolates from perioperative tissue samples from prostate cancer. Subtyping was based on recA, tly, and Tc12S sequences.

The MLST analysis identified 23 sequence types and displayed a superior ability to discriminate P. acnes isolates compared to DiversiLab and the subtyping. The highest discriminatory index was found when using seven genes. DiversiLab was better able to differentiate the isolates compared to the MLST clonal complexes of sequence types.

Our results suggest that DiversiLab can be useful as a rapid typing tool for initial discrimination of P. acnes isolates. When better discrimination is required, such as for investigations of the heterogeneity of P. acnes isolates and its involvement in different pathogenic processes, the present MLST protocol is valuable.

Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 18, no 4, p. 392-399
Keywords [en]
Propionibacterium acnes; Multilocus sequence typing (MLST); DiversiLab; Discrimination index; Molecular epidemiology
National Category
Microbiology in the medical area
Research subject
Microbiology
Identifiers
URN: urn:nbn:se:oru:diva-25346DOI: 10.1016/j.anaerobe.2012.04.015ISI: 000307688500003PubMedID: 22609518Scopus ID: 2-s2.0-84864371234OAI: oai:DiVA.org:oru-25346DiVA, id: diva2:547071
Note

Funding Agencies:

Örebro County Council Research Committee

Foundation for Medical Research at Orebro University Hospital, Sweden 

Available from: 2012-08-27 Created: 2012-08-27 Last updated: 2018-04-16Bibliographically approved
In thesis
1. Infection induced chronic inflammation and it's association with prostate cancer initiation and progression
Open this publication in new window or tab >>Infection induced chronic inflammation and it's association with prostate cancer initiation and progression
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

An association between cancer development and inflammation has long been suggested. Approximately 20% of all human cancers in adults are assumed to result from chronic inflammation. The aim of this thesis was to investigate if infection-induced chronic inflammation plays a role in prostate carcinogenesis.

Our results revealed a greater infiltration of the bacterium Propionibacterium acnes in the prostate tissue obtained from men with prostate cancer compared to men without any histological evidence of the disease. These findings indicate that prostate cancer could potentially be included in the list of cancers with an infectious etiology.

Further, we investigated whether chronic inflammation has a role in disease progression. Our results demonstrated that men with lethal prostate cancer had pronounced infiltration of immune cells with suppressive function of the anti-tumor immune response compared to men with a more indolent prostate cancer.

Confirmation of our results may open up avenues for targeted prostate cancer treatment by offering men with chronic inflammation alternative therapies such as anti-inflammatory drugs. If the involvement of P. acnes in prostate cancer development is replicated in other studies, vaccination therapies may be feasible. To further individualize prostate cancer therapy, bolstering the anti-tumor immune response in order to reduce tumor progression may be determined to be advantageous for some patients.

Place, publisher, year, edition, pages
Örebro: Örebro universitet, 2013. p. 65
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 86
Keywords
Prostate cancer, chronic inflammation, CD4 helper T cells, CD8 cytotoxic T cells, regulatory T cells, Propionibacterium acnes
National Category
Medical and Health Sciences
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-28466 (URN)978-91-7668-920-2 (ISBN)
Public defence
2013-05-31, Wilandersalen, Universitetssjukhuset i Örebro, Södra Grev Rosengatan 18, 703 62 Örebro, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2013-03-25 Created: 2013-03-25 Last updated: 2017-10-17Bibliographically approved

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Davidsson, SabinaSöderquist, BoElgh, FredrikAndrén, OveUnemo, Magnus

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