Multilocus sequence typing and repetitive-sequence-based PCR (DiversiLab) for molecular epidemiological characterization of Propionibacterium acnes isolates of heterogeneous originVisa övriga samt affilieringar
2012 (Engelska)Ingår i: Anaerobe, ISSN 1075-9964, E-ISSN 1095-8274, Vol. 18, nr 4, s. 392-399Artikel i tidskrift (Refereegranskat) Published
Abstract [en]
Propionibacterium acnes is a gram-positive bacillus predominantly found on the skin. Although it is considered an opportunistic pathogen it is also been associated with severe infections. Some specific P. acnes subtypes are hypothesized to be more prone to cause infection than others. Thus, the aim of the present study was to investigate the ability to discriminate between P. acnes isolates of a refined multilocus sequence typing (MLST) method and a genotyping method, DiversiLab, based on repetitive-sequence-PCR technology.
The MLST and DiversiLab analysis were performed on 29 P. acnes isolates of diverse origins; orthopedic implant infections, deep infections following cardiothoracic surgery, skin, and isolates from perioperative tissue samples from prostate cancer. Subtyping was based on recA, tly, and Tc12S sequences.
The MLST analysis identified 23 sequence types and displayed a superior ability to discriminate P. acnes isolates compared to DiversiLab and the subtyping. The highest discriminatory index was found when using seven genes. DiversiLab was better able to differentiate the isolates compared to the MLST clonal complexes of sequence types.
Our results suggest that DiversiLab can be useful as a rapid typing tool for initial discrimination of P. acnes isolates. When better discrimination is required, such as for investigations of the heterogeneity of P. acnes isolates and its involvement in different pathogenic processes, the present MLST protocol is valuable.
Ort, förlag, år, upplaga, sidor
Elsevier, 2012. Vol. 18, nr 4, s. 392-399
Nyckelord [en]
Propionibacterium acnes; Multilocus sequence typing (MLST); DiversiLab; Discrimination index; Molecular epidemiology
Nationell ämneskategori
Mikrobiologi inom det medicinska området
Forskningsämne
Mikrobiologi
Identifikatorer
URN: urn:nbn:se:oru:diva-25346DOI: 10.1016/j.anaerobe.2012.04.015ISI: 000307688500003PubMedID: 22609518Scopus ID: 2-s2.0-84864371234OAI: oai:DiVA.org:oru-25346DiVA, id: diva2:547071
Anmärkning
Funding Agencies:
Örebro County Council Research Committee
Foundation for Medical Research at Orebro University Hospital, Sweden
2012-08-272012-08-272024-03-04Bibliografiskt granskad
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