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Urinary Thymine Dimer as a Biomarker for Ultraviolet Radiation Exposure
Örebro University, School of Science and Technology.
2013 (English)Independent thesis Advanced level (degree of Master (Two Years)), 30 credits / 45 HE creditsStudent thesis
Abstract [en]

The incidence of skin cancer is growing rapidly among peoples of Caucasian origin. Increased exposure to ultraviolet radiation (UVR) from the sun and artificial sources are thought to be the main causative factor for this increase. UVR causes DNA lesions in skin cells, such us thymine dimers (T=T). Studies showed that after skin cells are repaired, T=T is excreted in the urine in significant amounts. The main aim of this study was to evaluate the effect of spending one week in the sunny island of Tenerife on the levels of urinary T=T. In addition the associations with other parameters like UVR dose, serum vitamin D level, pigment protection factor (PPF), erythema of the skin and time spent outdoors were analyzed. Urine and blood samples were collected from 20 participants in Barcelona, Spain, prior to their departure to Tenerife and immediately after return. Urine samples were purified and analyzed by HPLC-coupled 32P-postlabelling method to determine T=T levels and blood samples were used to determine vitamin D levels in serum. UVR exposure was measured using personal dosimeters. The area of skin exposed and time spent outdoors were reported by the participants. The other parameters, which are PPF and erythema of the skin, were also measured before and after the exposure period. Out of the 20 participants, nineteen showed a rise in T=T levels and serum vitamin D levels after the sunny vacation. Although anticipated, no correlation was found between T=T levels and UVR dose which might be due to a limited number of participants. However, several associations were found between the other study variables and an increase in many of the parameters was observed after the vacation. In conclusion we showed that T=T is a sensitive biomarker of exposure to UVR that can be applied in these types of field studies.

Place, publisher, year, edition, pages
2013. , p. 20
Keyword [en]
Skin cancer, Vitamin D, Cyclobutane pyrimidine dimers
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-30354OAI: oai:DiVA.org:oru-30354DiVA, id: diva2:643760
Subject / course
Biology
Uppsok
Life Earth Science
Supervisors
Examiners
Available from: 2013-08-28 Created: 2013-08-28 Last updated: 2017-10-17Bibliographically approved

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CiteExportLink to record
Permanent link

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Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf