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The role of calcium, NF-κB and NFAT in the regulation of CXCL8 and IL-6 expression in Jurkat T-cells
Örebro University, School of Science and Technology.
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-7957-0310
Örebro University, School of Science and Technology.ORCID iD: 0000-0001-7336-6335
2013 (English)In: International Journal of Biochemistry and Molecular Biology, ISSN 2152-4114, Vol. 4, no 3, p. 150-156Article in journal (Refereed) Published
Abstract [en]

T-cells play an important role in host immunity against invading pathogens. Determining the underlying regulatory mechanisms will provide a better understanding of T-cell-derived immune responses. In this study, we have shown the differential regulation of IL-6 and CXCL8 by NF-κB and NFAT in Jurkat T-cells, in response to PMA, heat killed Escherichia coli and calcium. CXCL8 was closely associated with the activation pattern of NFAT, while IL-6 expression was associated with NF-κB. Furthermore, increasing the intracellular Ca(2+) concentration by calcium ionophore treatment of the cells resulted in NFAT induction without affecting the NF-κB activity. Interestingly, NF-κB activation by heat killed E. coli, as well as CXCL8 and IL-6 expression was significantly suppressed following addition of the calcium ionophore. This indicates that calcium plays an important role in regulating protein trafficking and T-cell signalling, and the subsequent inflammatory gene expression infers an involvement of NFAT in CXCL8 regulation.Understanding these regulatory patterns provide clarification of conditions that involve altered intracellular signalling leading to T-cell-derived cytokine expression.

Place, publisher, year, edition, pages
2013. Vol. 4, no 3, p. 150-156
National Category
Natural Sciences Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-32847PubMedID: 24049670Scopus ID: 2-s2.0-84884266912OAI: oai:DiVA.org:oru-32847DiVA, id: diva2:681220
Funder
Knowledge FoundationAvailable from: 2013-12-19 Created: 2013-12-19 Last updated: 2019-04-03Bibliographically approved

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Khalaf, HazemJass, JanaOlsson, Per-Erik

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