oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Activation of NF-kappa B Protein Prevents the Transition from Juvenile Ovary to Testis and Promotes Ovarian Development in Zebrafish
Örebro University, School of Science and Technology.ORCID iD: 0000-0003-3302-7106
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
Baylor College of Medicine, Houston, USA.
Temasek Life Sciences Laboratory, Singapore, Singapore.
Show others and affiliations
2012 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 287, no 45, p. 37926-37938Article in journal (Refereed) Published
Abstract [en]

Testis differentiation in zebrafish involves juvenile ovary to testis transformation initiated by an apoptotic wave. The molecular regulation of this transformation process is not fully understood. NF-kappa B is activated at an early stage of development and has been shown to interact with steroidogenic factor-1 in mammals, leading to the suppression of anti-Mullerian hormone (Amh) gene expression. Because steroidogenic factor-1 and Amh are important for proper testis development, NF-kappa B-mediated induction of anti-apoptotic genes could, therefore, also play a role in zebrafish gonad differentiation. The aim of this study was to examine the potential role of NF-kappa B in zebrafish gonad differentiation. Exposure of juvenile zebrafish to heat-killed Escherichia coli activated the NF-kappa B pathways and resulted in an increased ratio of females from 30 to 85%. Microarray and quantitative real-time-PCR analysis of gonads showed elevated expression of NF-kappa B-regulated genes. To confirm the involvement of NF-kappa B-induced anti-apoptotic effects, zebrafish were treated with sodium deoxycholate, a known inducer of NF-kappa B or NF-kappa B activation inhibitor (NAI). Sodium deoxycholate treatment mimicked the effect of heat-killed bacteria and resulted in an increased proportion of females from 25 to 45%, whereas the inhibition of NF-kappa B using NAI resulted in a decrease in females from 45 to 20%. This study provides proof for an essential role of NF-kappa B in gonadal differentiation of zebrafish and represents an important step toward the complete understanding of the complicated process of sex differentiation in this species and possibly other cyprinid teleosts as well.

Place, publisher, year, edition, pages
The American Society for Biochemistry and Molecular Biology , 2012. Vol. 287, no 45, p. 37926-37938
National Category
Developmental Biology
Research subject
Biology
Identifiers
URN: urn:nbn:se:oru:diva-32903DOI: 10.1074/jbc.M112.386284ISI: 000310642200028Scopus ID: 2-s2.0-84868315208OAI: oai:DiVA.org:oru-32903DiVA, id: diva2:683130
Funder
Swedish Research CouncilKnowledge Foundation
Note

Funding Agency: Orebro University; Agri-Food and Veterinary Authority; Temasek Life Sciences Laboratory, Singapore (se även Forskningsfinansiärer)

Available from: 2014-01-02 Created: 2014-01-02 Last updated: 2018-11-29Bibliographically approved
In thesis
1. Molecular mechanisms of zebrafish sex differentiation and sexual behavior
Open this publication in new window or tab >>Molecular mechanisms of zebrafish sex differentiation and sexual behavior
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Örebro: Örebro university, 2015. p. 65
Series
Örebro Studies in Life Science ; 11
Keywords
NFκB, prostaglandin, retinoic acid, gonads, gene regulation, brain
National Category
Developmental Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:oru:diva-42118 (URN)9789175290621 (ISBN)
Public defence
2015-03-25, Hörsal B, Örebro universitet, Fakultetsgatan 1, Örebro, 10:00 (English)
Opponent
Supervisors
Available from: 2015-01-22 Created: 2015-01-21 Last updated: 2017-10-17Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus

Authority records BETA

Pradhan, AjayKhalaf, HazemKoskinen, JarnoKarlsson, MarieOlsson, Per-Erik

Search in DiVA

By author/editor
Pradhan, AjayKhalaf, HazemKoskinen, JarnoKarlsson, MarieOlsson, Per-Erik
By organisation
School of Science and TechnologySchool of Health and Medical Sciences, Örebro University, Sweden
In the same journal
Journal of Biological Chemistry
Developmental Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 564 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf