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Activation of NF-kappa B Protein Prevents the Transition from Juvenile Ovary to Testis and Promotes Ovarian Development in Zebrafish
Örebro universitet, Institutionen för naturvetenskap och teknik.ORCID-id: 0000-0003-3302-7106
Örebro universitet, Institutionen för hälsovetenskap och medicin.
Baylor College of Medicine, Houston, USA.
Temasek Life Sciences Laboratory, Singapore, Singapore.
Vise andre og tillknytning
2012 (engelsk)Inngår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 287, nr 45, s. 37926-37938Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Testis differentiation in zebrafish involves juvenile ovary to testis transformation initiated by an apoptotic wave. The molecular regulation of this transformation process is not fully understood. NF-kappa B is activated at an early stage of development and has been shown to interact with steroidogenic factor-1 in mammals, leading to the suppression of anti-Mullerian hormone (Amh) gene expression. Because steroidogenic factor-1 and Amh are important for proper testis development, NF-kappa B-mediated induction of anti-apoptotic genes could, therefore, also play a role in zebrafish gonad differentiation. The aim of this study was to examine the potential role of NF-kappa B in zebrafish gonad differentiation. Exposure of juvenile zebrafish to heat-killed Escherichia coli activated the NF-kappa B pathways and resulted in an increased ratio of females from 30 to 85%. Microarray and quantitative real-time-PCR analysis of gonads showed elevated expression of NF-kappa B-regulated genes. To confirm the involvement of NF-kappa B-induced anti-apoptotic effects, zebrafish were treated with sodium deoxycholate, a known inducer of NF-kappa B or NF-kappa B activation inhibitor (NAI). Sodium deoxycholate treatment mimicked the effect of heat-killed bacteria and resulted in an increased proportion of females from 25 to 45%, whereas the inhibition of NF-kappa B using NAI resulted in a decrease in females from 45 to 20%. This study provides proof for an essential role of NF-kappa B in gonadal differentiation of zebrafish and represents an important step toward the complete understanding of the complicated process of sex differentiation in this species and possibly other cyprinid teleosts as well.

sted, utgiver, år, opplag, sider
The American Society for Biochemistry and Molecular Biology , 2012. Vol. 287, nr 45, s. 37926-37938
HSV kategori
Forskningsprogram
Biologi
Identifikatorer
URN: urn:nbn:se:oru:diva-32903DOI: 10.1074/jbc.M112.386284ISI: 000310642200028Scopus ID: 2-s2.0-84868315208OAI: oai:DiVA.org:oru-32903DiVA, id: diva2:683130
Forskningsfinansiär
Swedish Research CouncilKnowledge Foundation
Merknad

Funding Agency: Orebro University; Agri-Food and Veterinary Authority; Temasek Life Sciences Laboratory, Singapore (se även Forskningsfinansiärer)

Tilgjengelig fra: 2014-01-02 Laget: 2014-01-02 Sist oppdatert: 2018-11-29bibliografisk kontrollert
Inngår i avhandling
1. Molecular mechanisms of zebrafish sex differentiation and sexual behavior
Åpne denne publikasjonen i ny fane eller vindu >>Molecular mechanisms of zebrafish sex differentiation and sexual behavior
2015 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
sted, utgiver, år, opplag, sider
Örebro: Örebro university, 2015. s. 65
Serie
Örebro Studies in Life Science ; 11
Emneord
NFκB, prostaglandin, retinoic acid, gonads, gene regulation, brain
HSV kategori
Forskningsprogram
Molekylärbiologi
Identifikatorer
urn:nbn:se:oru:diva-42118 (URN)9789175290621 (ISBN)
Disputas
2015-03-25, Hörsal B, Örebro universitet, Fakultetsgatan 1, Örebro, 10:00 (engelsk)
Opponent
Veileder
Tilgjengelig fra: 2015-01-22 Laget: 2015-01-21 Sist oppdatert: 2017-10-17bibliografisk kontrollert

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