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Amnion-derived mesenchymal stromal cells show a mesenchymal-epithelial phenotype in culture.
Örebro universitet, Institutionen för hälsovetenskap och medicin. Inst Cell Biol Histol & Embryol, Med Univ Graz, Graz, Austria.ORCID-id: 0000-0003-0466-1861
Inst Cell Biol Histol & Embryol, Med Univ Graz, Graz, Austria.
Inst Cell Biol Histol & Embryol, Med Univ Graz, Graz, Austria.
Inst Cell Biol Histol & Embryol, Med Univ Graz, Graz, Austria.
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2014 (Engelska)Ingår i: Cell and Tissue Banking, ISSN 1389-9333, E-ISSN 1573-6814, Vol. 15, nr 2, s. 193-198Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The amnionic membrane is a rich source of multipotent mesenchymal stromal cells (hAMSC), which are readily available and show a potential use in regenerative medicine and tissue engineering. Before these cells can be applied clinically, careful characterization is necessary, especially as primary cells are known to change their phenotype in culture. We analyzed the mesenchymal phenotype of hAMSC at different stages after isolation using immunohistochemistry. Shortly after isolation (1 day), 92 % (±7 %) of the hAMSC expressed the mesenchymal marker vimentin, 2 % (±1 %) stained for the epithelial marker cytokeratin-7 and 5 % (±4 %) co-expressed these markers. After 5 days, the double positive cells slightly increased to 7 % (±3 %), while exclusive expression of cytokeratin-7 or vimentin remained unchanged (1 % ± 2 % and 92 % ± 1 %, respectively). After the first passage, all attached cells were vimentin-positive, while 54 % (±9 %) co-expressed cytokeratin-7 and vimentin. Thus, we conclude that under culture, hAMSC adopt a hybrid mesenchymal-epithelial phenotype. It is also essential to perform microscopical examination during the first days after isolation to detect contaminations with human amnion-derived epithelial cells in cultures of hAMSC.

Ort, förlag, år, upplaga, sidor
Dordrecht: Springer Netherlands, 2014. Vol. 15, nr 2, s. 193-198
Nyckelord [en]
Amnion; Placenta; Mesenchymal stromal cells; Cytokeratin; Vimentin
Nationell ämneskategori
Medicin och hälsovetenskap
Forskningsämne
Medicin
Identifikatorer
URN: urn:nbn:se:oru:diva-33863DOI: 10.1007/s10561-013-9415-8ISI: 000337051300003PubMedID: 24326460Scopus ID: 2-s2.0-84902551356OAI: oai:DiVA.org:oru-33863DiVA, id: diva2:698151
Anmärkning

Funding Agencies:

Franz-Lanyar-Foundation 339 349

Medical University of Graz

Tillgänglig från: 2014-02-20 Skapad: 2014-02-20 Senast uppdaterad: 2019-03-26Bibliografiskt granskad

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König, Julia

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