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Altered faecal and mucosal microbial composition in post-infectious irritable bowel syndrome patients correlates with mucosal lymphocyte phenotypes and psychological distress
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. (NGBI)ORCID iD: 0000-0003-4713-1772
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. (NGBI)ORCID iD: 0000-0003-3383-9219
Department of Microbiology, Wageningen University, Wageningen,The Netherlands.
Department of Microbiology, Wageningen University, Wageningen,The Netherlands.
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2015 (English)In: Alimentary Pharmacology and Therapeutics, ISSN 0269-2813, E-ISSN 1365-2036, Vol. 41, no 4, p. 342-351Article in journal (Refereed) Published
Abstract [en]

Background: A subset of irritable bowel syndrome (IBS) patients, denoted post-infectious IBS (PI-IBS), develop symptoms after an enteric infection. Bacterial dysbiosis and mucosal inflammation have been proposed to be involved in the pathophysiology of this entity.

Aim: To characterise the mucosal and faecal microbiota in PI-IBS, general IBS and healthy controls, and to investigate associations between the microbiota and the mucosal immune system.

Methods: Mucosal biopsies and faeces were collected from 13 PI-IBS patients, 19 general IBS patients and 16 healthy controls. Global bacterial composition was determined by generating 16S rRNA amplicons that were examined by phylogenetic microarray hybridisation, principal component and redundancy analysis. We correlated previously reported lymphocyte proportions with the microbiota.

Results: Faecal microbiota composition of PI-IBS patients differed significantly from both general IBS patients and healthy controls (P < 0.02). Both mucosal (P < 0.01) and faecal (P = 0.05) microbial diversity were reduced in PI-IBS compared to healthy controls. In the intraepithelial lymphocytes the previously published proportion of CD8+ CD45RA+ was negatively correlated with mucosal microbial diversity (P < 0.005). The previously published number of lamina propria lymphocytes was negatively correlated with mucosal microbial diversity (P < 0.05). Faecal microbial diversity was significantly negatively correlated with the Hospital Anxiety and Depression scale (P < 0.05).

Conclusions: We present data that distinguishes the intestinal microbiota of PI-IBS patients from that of both general IBS patients and HC. The microbial composition is significantly associated with the HADs score and alterations in lymphocyte subsets proportions.

Place, publisher, year, edition, pages
Hoboken, USA: Wiley-Blackwell, 2015. Vol. 41, no 4, p. 342-351
National Category
Cell and Molecular Biology Gastroenterology and Hepatology
Identifiers
URN: urn:nbn:se:oru:diva-39955DOI: 10.1111/apt.13055ISI: 000348731200002PubMedID: 25521822Scopus ID: 2-s2.0-84921438829OAI: oai:DiVA.org:oru-39955DiVA, id: diva2:774083
Note

Funding Agencies:

Medical Faculty, Orebro University

Spinoza award of the Netherlands Organization for Scientific Research

Gravity grant (SIAM) of the Netherlands Organization for Scientific Research

Available from: 2014-12-22 Created: 2014-12-22 Last updated: 2018-01-11Bibliographically approved
In thesis
1. Microbe-host interactions in post-infectious irritable bowel syndrome
Open this publication in new window or tab >>Microbe-host interactions in post-infectious irritable bowel syndrome
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Örebro: Örebro university, 2015. p. 97
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 114
Keywords
IBS, PI-IBS, Intestinal, Mucosa, Lymphocyte, Microbiota, Bacteria, Cytokine, Addaptive immune response, IL-13
National Category
Gastroenterology and Hepatology
Research subject
Medicine
Identifiers
urn:nbn:se:oru:diva-41109 (URN)978-91-7529-057-7 (ISBN)
Public defence
2015-02-27, Prismahuset, Hörsal 2, Örebro universitet, Fakultetsgatan 1, Örebro, 09:00 (English)
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Available from: 2015-01-13 Created: 2015-01-13 Last updated: 2017-10-17Bibliographically approved

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Sundin, JohannaRangel, IgnacioHultgren-Hörnquist, ElisabethBrummer, Robert-Jan

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