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Orally inoculated Escherichia coli strains colonize the gut and increase bacterial translocation after stress in rats
Department of Surgery, Karolinska Hospital, Stockholm.
Department of Surgery, Karolinska Hospital, Stockholm.
Department of Surgery, Karolinska Hospital, Stockholm.
Department of Surgery, Karolinska Hospital, Stockholm.
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2003 (English)In: Shock, ISSN 1073-2322, E-ISSN 1540-0514, Vol. 20, no 3, p. 251-6Article in journal (Refereed) Published
Abstract [en]

Coliforms are the members of the indigenous gut flora that most often translocate to mesenteric lymph nodes. Very few strains of Escherichia coli found in cecal contents of rats are able to translocate. The present study investigated the role of the composition of the gut flora for the occurrence of bacterial translocation. Two strains of E. coli (KI-C1 and KI-C2), previously shown to translocate in rats subjected to stress, were given by oral inoculation to rats lacking these strains. A biochemical fingerprinting method was used to identify bacteria in cecal contents, on cecal epithelium, and in mesenteric lymph nodes. In a challenge study, the inoculated E. coli strains were shown to colonize the rats and persist for up to 75 days in cecum. Subsequently, one group was starved for 24 h and a second group was subjected to experimental hemorrhage and then starved for 24 h before sampling for bacteriological analyses from blood, cecum, and mesenteric lymph nodes. Two parallel groups of rats served as controls and were not inoculated but otherwise received the same treatment before sampling. In the inoculated group, starved for 24 h, seven out of 11 rats showed translocation, whereas in the noninoculated group one of 11 rats showed translocation (P < 0.05). In groups subjected to hemorrhage and then starved for 24 h, 15/22 rats in the inoculated and 5/20 rats in the noninoculated group showed translocation (P < 0.01). These findings show that orally inoculated KI-C1 and KI-C2 strains can colonize the gut and can substantially increase bacterial translocation in rats subjected to mild and severe stress. The composition of the gut flora seems to be an underestimated factor in the pathophysiology of bacterial translocation.

Place, publisher, year, edition, pages
Philadelphia, USA: Lippincott Williams & Wilkins, 2003. Vol. 20, no 3, p. 251-6
Keywords [en]
Escherichia coli, translocating strains, biochemical fingerprinting, bacterial translocation, experimental hemorrage
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:oru:diva-40385DOI: 10.1097/01.shk.0000068324.41183.c2ISI: 000184841100009PubMedID: 12923497Scopus ID: 2-s2.0-2342453220OAI: oai:DiVA.org:oru-40385DiVA, id: diva2:777150
Available from: 2015-01-08 Created: 2015-01-08 Last updated: 2017-12-05Bibliographically approved

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