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Unlocking pathology archives for microRNA-profiling
Institute for Pathology, UKSH Campus Luebeck, Luebeck.
Institute for Pathology, UKSH Campus Luebeck, Luebeck.
Institute for Pathology, UKSH Campus Luebeck, Luebeck.
Institute for Pathology, UKSH Campus Luebeck, Luebeck.
Vise andre og tillknytning
2008 (engelsk)Inngår i: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 28, nr 1A, s. 119-23Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: MicroRNAs (miRNAs) are approximately 22 nucleotide long, non-coding RNAs that regulate gene expression by binding to the 3'-untranslated region of target mRNAs and also a variety of cellular processes. It has recently been established that dysregulation of miRNA expression can be detected in the majority of human cancers. A variety of high-throughput screening methods has been developed to identify dysregulated miRNA species in tumours. For retrospective clinical studies formalin-fixed, paraffin-embedded (FFPE) tissue is the most widely used material.

Materials and methods: The miRNA expression profiles of freshly frozen (CRYO) and FFPE tissues of seven tonsil and four liver samples were compared, using a qPCR-based assay, profiling 157 miRNA species.

Results: The significance of miRNA-profiles was barely influenced by FFPE treatment in both tissues and the variance induced by FFPE treatment was much smaller than the variance caused by biologically based differential expression.

Conclusion: FFPE material is well suited for miRNA profiling.

sted, utgiver, år, opplag, sider
Athens, Greece: International Institute of Anticancer Research, 2008. Vol. 28, nr 1A, s. 119-23
Emneord [en]
miRNA, FFPE, expression profiling
HSV kategori
Identifikatorer
URN: urn:nbn:se:oru:diva-40636ISI: 000253844200017PubMedID: 18383833Scopus ID: 2-s2.0-40549095349OAI: oai:DiVA.org:oru-40636DiVA, id: diva2:778025
Tilgjengelig fra: 2015-01-09 Laget: 2015-01-09 Sist oppdatert: 2018-01-30bibliografisk kontrollert

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