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Two-color microarray experiments. Technology and sources of variance.
Institut für Medizinische Biometrie und Statistik, Universität zu Lübeck, Lübeck, Germany.ORCID-id: 0000-0002-7173-5579
Institut für Medizinische Biometrie und Statistik, Universität zu Lübeck, Lübeck, Germany .
2005 (engelsk)Inngår i: Methods of Information in Medicine, ISSN 0026-1270, Vol. 44, nr 3, s. 400-4Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Objectives: Microarray gene expression experiments have a complex technical background. Knowledge about certain technical details is inevitable to judge alternatives for both experimental design and analysis. Here, we introduce the necessary details for the so-called two-color microarray experiments and review major sources of technical variance.

Methods: We follow the sequence of experimental steps during a typical two-color microarray gene expression experiment, stressing decisive points in the choice of technique, experimental handling and biophysical basics. We point out where technical variation is to be expected.

Results: Tissue storage, RNA extraction techniques, as well as the microarray hybridization represent major components of technical variance to be considered. Depending on the possibilities for access to the biomedical material under investigation, choice of amplification and labeling techniques can also be decisive to avoid additional technical variance. The two-color microarray experimental approach seeks to avoid a group of probe-level technical biases making use of the advantages of an incomplete block-design.

Conclusions: It is worth to know the major sources of technical variance during the typical experimental sequence, both for choice of experimental design and techniques of molecular biology, as well as for the understanding of quality control and normalization approaches. Here, early investments pay at the level of reduced technical variance, allowing for enhanced detection levels for the effects under investigation.

sted, utgiver, år, opplag, sider
Stuttgart, Germany: Schattauer Gmbh, 2005. Vol. 44, nr 3, s. 400-4
Emneord [en]
Agilent, chip, comparative hybridization, gene expression, microarray, technical variance
HSV kategori
Identifikatorer
URN: urn:nbn:se:oru:diva-40715ISI: 000230734600008PubMedID: 16113763Scopus ID: 2-s2.0-22944471092OAI: oai:DiVA.org:oru-40715DiVA, id: diva2:778523
Tilgjengelig fra: 2015-01-10 Laget: 2015-01-10 Sist oppdatert: 2018-01-30bibliografisk kontrollert

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