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Cytokine Response after Stimulation with Key Commensal Bacteria Differ in Post-Infectious Irritable Bowel Syndrome (PI-IBS) Patients Compared to Healthy Controls
Örebro universitet, Institutionen för hälsovetenskap och medicin.ORCID-id: 0000-0003-4713-1772
Örebro universitet, Institutionen för hälsovetenskap och medicin.ORCID-id: 0000-0003-3383-9219
Örebro universitet, Institutionen för hälsovetenskap och medicin.ORCID-id: 0000-0002-7173-5579
Örebro universitet, Institutionen för läkarutbildning.ORCID-id: 0000-0002-0362-0008
2015 (engelsk)Inngår i: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 10, nr 9, artikkel-id e0134836Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Background: Microbial dysbiosis and prolonged immune activation resulting in low-grade inflammation and intestinal barrier dysfunction have been suggested to be underlying causes of post-infectious irritable bowel syndrome (PI-IBS). The aim of this study was to evaluate the difference in cytokine response between mucosal specimens of PI-IBS patients and healthy controls (HC) after ex vivo stimulation with key anaerobic bacteria.

Methods: Colonic biopsies from 11 PI-IBS patients and 10 HC were stimulated ex vivo with the commensal bacteria Bacteroides ovatus, Ruminococcus gnavus, Akkermansia muciniphila, Subdoligranulum variabile and Eubacterium limosum, respectively. The cytokine release (IL-1 beta, IL-2, IL-8, IL-10, IL-13, IL-17, TNF-alpha and IFN-gamma) in stimulation supernatants was analyzed using the LUMINEX assay. Comparison of cytokine release between PI-IBS patients and healthy controls was performed taking both unstimulated and bacterially stimulated mucosal specimens into account.

Key Results: IL-13 release from mucosal specimens without bacterial stimulation was significantly lower in PI-IBS patients compared to HC (p < 0.05). After stimulation with Subdoligranulum variabile, IL-1 beta release from PI-IBS patients was significantly increased compared to HC (p < 0.05). Stimulation with Eubacterium limosum resulted in a significantly decreased IL-10 release in HC compared to PI-IBS patients (p < 0.05) and a tendency to decreased IL-13 release in HC compared to PI-IBS patients (p = 0.07).

Conclusions & Inferences: PI-IBS patients differ from HC with regard to cytokine release ex vivo after stimulation with selected commensal bacteria. Hence, our results support that the pathogenesis of PI-IBS comprises an altered immune response against commensal gut microbes.

sted, utgiver, år, opplag, sider
2015. Vol. 10, nr 9, artikkel-id e0134836
HSV kategori
Identifikatorer
URN: urn:nbn:se:oru:diva-46171DOI: 10.1371/journal.pone.0134836ISI: 000361601100007PubMedID: 26366730OAI: oai:DiVA.org:oru-46171DiVA, id: diva2:861859
Merknad

Funding Agency:

Medical Faculty, Örebro University

Tilgjengelig fra: 2015-10-19 Laget: 2015-10-19 Sist oppdatert: 2017-12-01bibliografisk kontrollert

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