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Antibacterial effects of lactobacillus and bacteriocin NC8 αβ on the periodontal pathogen Porphyromonas gingivalis
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. PEAS Institut AB, Söderleden 1, Linköping.
Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.
Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.
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(English)Manuscript (preprint) (Other academic)
National Category
Microbiology in the medical area
Research subject
Biomedicine
Identifiers
URN: urn:nbn:se:oru:diva-46730OAI: oai:DiVA.org:oru-46730DiVA, id: diva2:873472
Available from: 2015-11-24 Created: 2015-11-24 Last updated: 2018-01-10Bibliographically approved
In thesis
1. Development of novel tools for prevention and diagnosis of Porphyromonas gingivalis infection and periodontitis
Open this publication in new window or tab >>Development of novel tools for prevention and diagnosis of Porphyromonas gingivalis infection and periodontitis
2015 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Periodontitis is a chronic infectious disease causing inflammation and destruction of tooth-supporting structures eventually leading to tooth loss. Periodontitis is induced by different subgingival microorganisms in combination with an excessive and dysregulated immune-inflammatory response against this microbial challenge. The pathogenicity of the periodontal biofilm is highly dependent upon some "keystone" species of which Porphyromonas gingivalis is considered to be one of the most important pathogens. P. gingivalis expresses a broad range of virulence factors including lipopolysaccharides (LPS), fimbriae, adhesins, hemagglutinins and an array of proteolytic enzymes. Among these factors, cysteine proteases (gingipains) are of special importance both for the bacterial survival/proliferation and for the pathological outcome. Gingipains consist of arginine-specific proteases (RgpA and RgpB) and lysine-specific protease (Kgp). The major aim of this thesis was to develop and test novel methods for diagnosis and prevention of P. gingivalis infection and periodontitis. In study I, host lymphocytes were stimulated with P. gingivalis to develop antibodies in vitro and the anti-P. gingivalis antibodies were used for immunodetection in clinical samples. The in vitro method of antibody production developed during this study could be used for an efficient real-time detection of P. gingivalis infection and periodontitis, while the attenuating effects of the antibodies suggest a role in passive immunization to prevent periodontitis and its associated diseases. In study II, we have elucidated the properties and antimicrobial effects of different lactobacillus species and the two-peptide bacteriocin NC8 αβ on P. gingivalis. NC8 αβ was found to be efficient against P. gingivalis through bacterial binding followed by permeabilization of the membranes. Liposomal systems were acquired to verify membrane permeabilization by NC8 αβ. The antimicrobial activity of NC8 αβ was found to be rapid, potent and instant. In conclusion, soluble or immobilized NC8 αβ bacteriocins may be used to prevent P. gingivalis colonization and subsequent pathogenicity, and thus supplement the host immune system against invading pathogens associated with periodontitis.

Place, publisher, year, edition, pages
Örebro: Örebro University, 2015. p. 30
National Category
Microbiology in the medical area
Research subject
Biomedicine
Identifiers
urn:nbn:se:oru:diva-46712 (URN)
Available from: 2015-11-24 Created: 2015-11-23 Last updated: 2018-01-10Bibliographically approved

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Khalaf, HazemNakka, Sravya SowdaminiScherbak, NikolaiBengtsson, Torbjörn

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Khalaf, HazemNakka, Sravya SowdaminiScherbak, NikolaiBengtsson, Torbjörn
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School of Health and Medical Sciences, Örebro University, SwedenSchool of Science and TechnologySchool of Medicine, Örebro University, Sweden
Microbiology in the medical area

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