oru.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Does perfluorooctane sulfonate (PFOS) act as chemosensitizer in zebrafish embryos?
Aquatic Ecology and Toxicology Group, Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany.
Department Bioanalytical Ecotoxicology, UFZ-Helmholtz Centre for Environmental Research, Leipzig, Germany.
Department Bioanalytical Ecotoxicology, UFZ-Helmholtz Centre for Environmental Research, Leipzig, Germany.
Aquatic Ecology and Toxicology Group, Centre for Organismal Studies, University of Heidelberg, Heidelberg, Germany.
Visa övriga samt affilieringar
2016 (Engelska)Ingår i: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 548-549, s. 317-324Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Earlier studies have shown that perfluorooctane sulfonate (PFOS) increases the toxicity of other chemicals by enhancing their uptake by cells and tissues. The present study aimed at testing whether the underlying mechanism of enhanced uptake of chemicals by zebrafish (Danio rerio) embryos in the presence of PFOS is by interference of this compound with the cellular efflux transporter Abcb4. Modifications of uptake/clearance and toxicity of two Abcb4 substrates, the fluorescent dye rhodamine B (RhB) and vinblastine, by PFOS were evaluated using 24 and 48. h post-fertilization (hpf) embryos. Upon 90. min exposure of 24. hpf embryos to 1. μM RhB and different PFOS concentrations (3-300. μM) accumulation of RhB in zebrafish was increased by up to 11.9-fold compared to controls, whereas RhB increases in verapamil treatments were 1.7-fold. Co-administration of PFOS and vinblastine in exposures from 0 to 48. hpf resulted in higher vinblastine-caused mortalities in zebrafish embryos indicating increased uptake of this compound. Interference of PFOS with zebrafish Abcb4 activity was further studied using recombinant protein obtained with the baculovirus expression system. PFOS lead to a concentration-dependent decrease of the verapamil-stimulated Abcb4 ATPase activity; at higher PFOS concentrations (250, 500. μM), also the basal ATPase activity was lowered indicating PFOS to be an Abcb4 inhibitor. In exposures of 48. hpf embryos to a very high RhB concentration (200. μM), accumulation of RhB in embryo tissue and adsorption to the chorion were increased in the presence of 50 or 100. μM PFOS. In conclusion, the results indicate that PFOS acts as inhibitor of zebrafish Abcb4; however, the exceptionally large PFOS-caused effect amplitude of RhB accumulation in the 1. μM RhB experiments and the clear PFOS effects in the experiments with 200. μM RhB suggest that an additional mechanism appears to be responsible for the potential of PFOS to enhance uptake of Abcb4 substrates.

Ort, förlag, år, upplaga, sidor
Elsevier, 2016. Vol. 548-549, s. 317-324
Nyckelord [en]
Abcb4; Chemosensitization; Environment-tissue barrier; Multixenobiotic resistance (MXR); P-glycoprotein; Perfluorooctane sulfonate; Zebrafish embryo
Nationell ämneskategori
Miljövetenskap
Forskningsämne
Miljövetenskap
Identifikatorer
URN: urn:nbn:se:oru:diva-48136DOI: 10.1016/j.scitotenv.2015.12.089ISI: 000370246000033PubMedID: 26803730Scopus ID: 2-s2.0-84955300538OAI: oai:DiVA.org:oru-48136DiVA, id: diva2:901873
Anmärkning

Funding Agencies:

Landesgraduiertenforderung (LGFG)

University of Heidelberg

Ministry of Science, Research

Arts of Baden-Wurttemberg

German Federal Environmental Foundation (DBU)

Tillgänglig från: 2016-02-09 Skapad: 2016-02-09 Senast uppdaterad: 2017-11-30Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Personposter BETA

Engwall, MagnusKeiter, Steffen

Sök vidare i DiVA

Av författaren/redaktören
Engwall, MagnusKeiter, Steffen
Av organisationen
Institutionen för naturvetenskap och teknik
I samma tidskrift
Science of the Total Environment
Miljövetenskap

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 545 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf