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An effective clean-up technique for GC/EI-HRMS determination of developmental neurotoxicants in human breast milk
Research Centre for Toxic Compounds in the Environment RECETOX), Faculty of Science, Masaryk University, Kamenice, Brno, Czech Republic.
Research Centre for Toxic Compounds in the Environment RECETOX), Faculty of Science, Masaryk University, Kamenice, Brno, Czech Republic.
Örebro University, School of Science and Technology, Örebro University, Sweden. Research Centre for Toxic Compounds in the Environment RECETOX), Faculty of Science, Masaryk University, Kamenice, Brno, Czech Republic. (Man-Technology-Environment Research Centre)ORCID iD: 0000-0003-1733-8334
Research Centre for Toxic Compounds in the Environment RECETOX), Faculty of Science, Masaryk University, Kamenice, Brno, Czech Republic.
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2017 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 409, no 5, 1311-1322 p.Article in journal (Refereed) Published
Abstract [en]

The increasing number of children suffering from developmental disorders has raised questions regarding their association with the presence of environmental contaminants in mothers and children. We therefore developed a new method for the determination of 78 proven and potential developmental neurotoxicants, including polychlorinated biphenyls, legacy pesticides, pyrethroids, and old and new halogenated flame retardants in breast milk. The essential part of sample preparation was dialysis as a non-destructive clean-up step which was newly used at 10 °C and showed more efficient lipid removal (up to 96%) than the conventional methods such as gel permeation chromatography or freezing-lipid filtration and thus ensured low limits of detection (LOD) by reducing the sample volume prior to injection. Next advantages were significant solvent reduction and no risk of sample cross-contamination. Gas chromatography coupled with high resolution mass spectrometry (GC-HRMS) was subsequently used for the separation and compound quantification. The method was validated using breast milk samples fortified with the analyzed compounds. Recoveries for most of the compounds ranged from 63 to 121% with a relative standard deviation of 2-25%, and LODs ranged between 0.001 and 0.87 ng g(-1) lipid weight. The method was applied to breast milk samples from a Dutch birth cohort where 35 out of the 78 compounds were quantified in more than 60% of the samples. For novel flame retardants, the method provides unique results regarding their occurrence in human matrices in Europe. Overall, the analysis of a complex mixture of developmental neurotoxicants could be useful for the assessment of the influence of the studied compounds to child health and development. Graphical abstract Flow diagram of the method and levels of the developmental neurotoxicants in Dutch human milk samples.

Place, publisher, year, edition, pages
Heidelberg, Germany: Springer Berlin/Heidelberg, 2017. Vol. 409, no 5, 1311-1322 p.
Keyword [en]
Developmental neurotoxicants, dialysis, GC-MS, human breast milk, non-destructive clean-up
National Category
Environmental Health and Occupational Health Biochemistry and Molecular Biology Chemical Sciences
Identifiers
URN: urn:nbn:se:oru:diva-53491DOI: 10.1007/s00216-016-0059-yISI: 000392622800014PubMedID: 27830313Scopus ID: 2-s2.0-84994461723OAI: oai:DiVA.org:oru-53491DiVA: diva2:1046444
Funder
EU, FP7, Seventh Framework Programme, 282957
Note

Funding Agencies:

"Employment of Best Young Scientists for International Cooperation Empowerment" - European Social Fund CZ.1.07/2.3.00/30.0037

Sate budget of the Czech Republic

Czech Ministry of Education, Youth and Sports LO1214  LM 2015051

Available from: 2016-11-14 Created: 2016-11-14 Last updated: 2017-03-06Bibliographically approved

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Kukučka, Petr
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School of Science and Technology, Örebro University, Sweden
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