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Analysis of the Langat Virus Genome in Persistent Infection of an Ixodes scapularis Cell Line
Biology of Vector-Borne Viruses Section, Laboratory of Virology, National Institutes of Health, Hamilton, MT, USA.
Örebro University, School of Medical Sciences. Biology of Vector-Borne Viruses Section, Laboratory of Virology, National Institutes of Health, Hamilton, MT, USA.
Biology of Vector-Borne Viruses Section, Laboratory of Virology, National Institutes of Health, Hamilton, MT, USA.
Genomics Unit, Research Technologies Branch, Hamilton, MT, USA.
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2016 (English)In: Viruses, ISSN 1999-4915, E-ISSN 1999-4915, Vol. 8, no 9, 252Article in journal (Refereed) Published
Abstract [en]

Tick-borne flaviviruses (TBFVs) cause a broad spectrum of disease manifestations ranging from asymptomatic to mild febrile illness and life threatening encephalitis. These single-stranded positive-sense (ss(+)) RNA viruses are naturally maintained in a persistent infection of ixodid ticks and small-medium sized mammals. The development of cell lines from the ixodid ticks has provided a valuable surrogate system for studying the biology of TBFVs in vitro. When we infected ISE6 cells, an Ixodes scapularis embryonic cell line, with Langat virus (LGTV) we observed that the infection proceeded directly into persistence without any cytopathic effect. Analysis of the viral genome at selected time points showed that no defective genomes were generated during LGTV persistence by 10 weeks of cell passage. This was in contrast to LGTV persistence in 293T cells in which defective viral genomes are detectable by five weeks of serial cell passage. We identified two synonymous nucleotide changes i.e., 1893AC (29% of 5978 reads at 12 h post infection (hpi)) and 2284TA (34% of 4191 reads at 12 hpi) in the region encoding for the viral protein E. These results suggested that the mechanisms supporting LGTV persistence are different between tick and mammalian cells.

Place, publisher, year, edition, pages
Basel: MDPI AG , 2016. Vol. 8, no 9, 252
Keyword [en]
Langat virus genome, tick-borne flavivirus, persistent infection, Ixodes scapularis, ISE6 cells, deep-sequencing
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:oru:diva-53517DOI: 10.3390/v8090252ISI: 000385396800012ScopusID: 2-s2.0-84987846223OAI: oai:DiVA.org:oru-53517DiVA: diva2:1046819
Note

Funding Agency:

Division of Intramural Research of the NIAID, NIH

Available from: 2016-11-15 Created: 2016-11-15 Last updated: 2016-11-15Bibliographically approved

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