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Photodynamic UVA-riboflavin bacterial elimination in antibiotic-resistant bacteria
Örebro University, School of Medical Sciences. Department of Ophthalmology .
Örebro University, School of Medical Sciences. Clinical Research Centre.
2016 (English)In: Clinical and Experimental Ophthalmology, ISSN 1442-6404, E-ISSN 1442-9071, Vol. 44, no 7, 582-586 p.Article in journal (Refereed) Published
Abstract [en]

Background: To evaluate the bactericidal effect of clinical ultraviolet A (UVA) settings used in photoactivated chromophore for infectious keratitis (PACK)-collagen cross-linking (CXL) in antibiotic-resistant and non-resistant bacterial strains.

Methods: Well-characterized bacterial strains from clinical isolates, without and with antibiotic resistance, were studied in a pairwise comparison. The evaluated pathogens were Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis. Bacteria were dispersed in PBS and diluted to a concentration of approximately 4x10(5)/ml. Riboflavin was added to a concentration of 0.01%. By spreading the solution on a microscope slide, a fluid film layer, with a thickness of around 400mm, was formed and UVA exposure followed. Eight separate exposures were made for each strain (n=8). The degree of elimination in resistant and non-resistant pathogens was compared.

Results: The bactericidal efficacy of exposure differed between the tested microorganisms, and the mean elimination ranged between 60 and 92%, being most extensive in both of the evaluated Pseudomonas strains and least in the E. faecalis strains. Similar reductions were seen in antibiotic-resistant and non-resistant strains, with the exception of S. aureus, in which the resistant strain metchicillin-resistant Staphylococcus aureus (MRSA) was eradicated in a greater extent than the non-resistant strain (P=0.030).

Conclusion: UVA-riboflavin settings used in PACK-CXL are effective in reducing both antibiotic-resistant and non-resistant bacteria. Antibiotic resistance does not appear to be protective against the photooxidative exposure.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2016. Vol. 44, no 7, 582-586 p.
Keyword [en]
bacteria, resistance, riboflavin, ultraviolet, UVA
National Category
URN: urn:nbn:se:oru:diva-53635DOI: 10.1111/ceo.12723ISI: 000386685300009PubMedID: 26867998ScopusID: 2-s2.0-84963632197OAI: oai:DiVA.org:oru-53635DiVA: diva2:1050093

Funding Agency:

Örebro University Hospital (Sweden) OLL-294081  OLL-393791

Available from: 2016-11-28 Created: 2016-11-25 Last updated: 2016-11-28Bibliographically approved

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Makdoumi, KarimBäckman, Anders
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