oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Interleukin-1-mediated effects of normal oral keratinocytes and head and neck squamous carcinoma cells on extracellular matrix related gene expression in fibroblasts
Department of Surgical Sciences, Plastic Surgery, Uppsala University, Uppsala, Sweden.
Clinical Research Center, University Hospital, Örebro, Sweden.
Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Örebro University, School of Health and Medical Sciences, Örebro University, Sweden. Clinical Research Center, University Hospital, Örebro, Sweden.
Show others and affiliations
2012 (English)In: Oral Oncology, ISSN 1368-8375, E-ISSN 1879-0593, Vol. 48, no 12, p. 1236-1241Article in journal (Refereed) Published
Abstract [en]

Objectives: The composition of tumor stroma and the activity of tumor associated fibroblasts are important for tumor growth. Interactions between carcinoma cells and fibroblasts regulate the turnover of extracellular matrix (ECM). Here, the in vitro effects of oral squamous cell carcinoma (SCC) cells (UT-SCC-30 and UT-SCC-87) on fibroblast expression of genes for ECM components and connective tissue growth factor (CTGF/CCN2), were compared to those of normal oral keratinocytes (NOK).

Materials and Methods: Cocultures with fibroblasts in collagen gels and keratinocytes with the two cell types separated by a semi permeable membrane were used, and relative gene expression was measured with real-time PCR.

Results: All investigated genes were regulated by NOK and the SCCs. The downregulation of pro-collagens alpha 1(I) and alpha 1(III) was more pronounced in cocultures with NOK, while the expression of CCN2 and fibronectin was downregulated by both NOK and the SCCs to a similar extent. UT-SCC-87, but not UT-SCC-30, secreted significantly more IL-1 alpha than NOK. A recombinant interleukin-1 receptor antagonist reversed many of the observed effects on fibroblast gene expression suggesting involvement of IL-1 in cocultures with NOK as well as with SCCs.

Conclusion: The observed differential effects on fibroblast gene expression suggest that NOK are more antifibrotic compared to UT-SCC-30 and UT-SCC-87. These findings may contribute to a better understanding of the mechanisms behind ECM turnover in tumors. (C) 2012 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 48, no 12, p. 1236-1241
Keywords [en]
Cocultures, Extracellular matrix, Interleukin-1 alpha, Tumor stroma
National Category
Cancer and Oncology Dentistry
Research subject
Oncology
Identifiers
URN: urn:nbn:se:oru:diva-55677DOI: 10.1016/j.oraloncology.2012.06.013ISI: 000311151200007PubMedID: 22796477Scopus ID: 2-s2.0-84869504827OAI: oai:DiVA.org:oru-55677DiVA, id: diva2:1074005
Funder
Swedish Cancer Society, 090592
Note

Funding Agency:

Thureus Foundation R121

Available from: 2017-02-14 Created: 2017-02-14 Last updated: 2017-11-29Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Ivarsson, Mikael
By organisation
School of Health and Medical Sciences, Örebro University, Sweden
In the same journal
Oral Oncology
Cancer and OncologyDentistry

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 140 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf