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Human skeletal muscle cell differentiation is associated with changes in myogenic markers and enhanced insulin-mediated MAPK and PKB phosphorylation
Department of Surgical Science, Karolinska Institutet, Stockholm, Sweden; Dept. of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Integrative Physiology, Dept. of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Department of Surgical Science, Karolinska Institutet, Stockholm, Sweden; Dept. of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Orebro University Hospital. Department of Surgical Science, Karolinska Institutet, Stockholm, Sweden.
Department of Surgical Science, Karolinska Institutet, Stockholm, Sweden Dept. of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
2004 (English)In: Acta Physiologica Scandinavica, ISSN 0001-6772, E-ISSN 1365-201X, Vol. 180, no 4, 395-403 p.Article in journal (Refereed) Published
Abstract [en]

AIM: We hypothesized that myogenic differentiation of HSMC would yield a more insulin responsive phenotype.

METHODS: We assessed expression of several proteins involved in insulin action or myogenesis during differentiation of primary human skeletal muscle cultures (HSMC).

RESULTS: Differentiation increased creatine kinase activity and expression of desmin and myocyte enhancer factor (MEF)2C. No change in expression was observed for big mitogen-activated protein kinase (BMK1/ERK5), MEF2A, insulin receptor (IR), hexokinase II, and IR substrates 1 and 2, while expression of glycogen synthase, extracellular signal-regulated kinase 1 and 2 (ERK1/2 MAP kinase) and the insulin responsive aminopeptidase increased after differentiation. In contrast to protein kinase B (PKB)a, expression of (PKB)b increased, with differentiation. Both basal and insulin-stimulated PI 3-kinase activity increased with differentiation. Insulin-mediated phosphorylation of PKB and ERK1/2 MAP kinase increased after differentiation.

CONCLUSION: Components of the insulin-signalling machinery are expressed in myoblast and myotube HSMC; however, insulin responsiveness to PKB and ERK MAP kinase phosphorylation increases with differentiation.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2004. Vol. 180, no 4, 395-403 p.
Keyword [en]
Akt; protein kinase B; cell culture; ERK mitogen-activated protein kinase
National Category
Psychology
Research subject
Psychology
Identifiers
URN: urn:nbn:se:oru:diva-55759DOI: 10.1111/j.1365-201X.2004.01259.xISI: 000220306100009PubMedID: 15030381Scopus ID: 2-s2.0-194250226OAI: oai:DiVA.org:oru-55759DiVA: diva2:1074536
Available from: 2017-02-15 Created: 2017-02-15 Last updated: 2017-04-03Bibliographically approved

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