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CD138 and CD31 Double-Positive Cells Comprise the Functional Antibody-Secreting Plasma Cell Compartment in Primate Bone Marrow
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden; Immunology Division, Garvan Institute of Medical Research, Darlinghurst, NSW, Australia.
Division of Clinical Research Centre, Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden.
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2016 (English)In: Frontiers in Immunology, ISSN 1664-3224, E-ISSN 1664-3224, Vol. 7, 242Article in journal (Refereed) Published
Abstract [en]

Plasma cells (PCs) are defined as terminally differentiated B cells that secrete large amounts of immunoglobulin (Ig). PCs that reside in the bone marrow (BM) are responsible for maintaining long-term antibody (Ab) responses after infection and vaccination, while PCs present in the blood are generally short-lived. In rhesus macaques, a species frequently used for the evaluation of human vaccines, B cells resemble those found in humans. However, a detailed characterization of BM-resident rhesus PC phenotype and function is lacking. Here, we examined Ig secretion of distinct rhesus CD138+ populations by B cell ELISpot analysis to couple phenotype with function. We demonstrate that the CD20low/-CD138+CD31+ BM population was highly enriched for antibody-secreting cells with IgG being the predominant isotype (60%), followed by IgA (33%) and IgM (7%). Transmission electron microscopy analysis confirmed PC enrichment in the CD20low/-CD138+CD31+ population with cells containing nuclei with "spokes of a wheel" chromatin structure and prominent rough endoplasmic reticulum. This panel also stained human BM PCs and allowed a clear distinction between BM PCs and short-lived peripheral PCs, providing an improved strategy to isolate PCs from rhesus BM for further analysis.

Place, publisher, year, edition, pages
Lausanne, Switzerland: Frontiers Media S.A., 2016. Vol. 7, 242
National Category
Medical and Health Sciences Immunology
Identifiers
URN: urn:nbn:se:oru:diva-55795DOI: 10.3389/fimmu.2016.00242ISI: 000378494700001PubMedID: 27446073Scopus ID: 2-s2.0-84977560080OAI: oai:DiVA.org:oru-55795DiVA: diva2:1074599
Note

Funiding Agencies:

Karolinska Institutet KID grant 

Swedish Research Council international postdoc fellowship 

NIH P01 grant (HIVRAD) 

International AIDS Initiative (IAVI) grant

medical internship combined with research (Forskar-AT) grant 

Forskar-AT grant 

Available from: 2017-02-15 Created: 2017-02-15 Last updated: 2017-03-09Bibliographically approved

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