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The role of toll-like and protease-activated receptors and associated intracellular signaling in Porphyromonas gingivalis-infected gingival fibroblasts
Örebro University, School of Medical Sciences.
Örebro University, School of Medical Sciences.
Örebro University, School of Medical Sciences.
Örebro University, School of Medical Sciences.
2017 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 125, no 2, 157-169 p.Article in journal (Refereed) Published
Abstract [en]

Porphyromonas gingivalis, which is considered a keystone agent in periodontitis, has evolved elaborate mechanisms to grow and survive in a hostile milieu. The gingival fibroblast is the major cell type in the gingiva and is considered to be important in the periodontitis-associated inflammation. As a part of the innate immune response, they produce cytokines such as CXCL8 and interleukin (IL)-6 which are believed to contribute to the destruction of the tooth-supporting tissues. This study investigates how the expression of protease-activated receptors (PAR1, PAR2) and toll-like receptors (TLR2, TLR4) changes with P. gingivalis exposure and how silencing of one receptor affects the expression of the other receptors. The importance of protein kinase C (PKC) and p38 in the regulation of CXCL8 and IL-6 was also examined. Receptors were knockdown with small-interfering RNA. PKC or p38 was blocked prior to stimulation with P. gingivalis. Fibroblasts were able to compensate for PAR1 knockdown with increased expression of PAR2. PKC and p38 were involved in the regulation of P. gingivalis-induced CXCL8 and IL-6. Our results indicate that PAR1 and PAR2 could be implicated in periodontitis and that PKC and P38 play a role in the inflammatory response in P. gingivalis-infected gingival fibroblasts.

Place, publisher, year, edition, pages
Hoboken, USA: Wiley-Blackwell Publishing Inc., 2017. Vol. 125, no 2, 157-169 p.
Keyword [en]
Porphyromonas gingivalis, gingival fibroblasts, protease-activated receptors, toll-like receptors, cell signaling
National Category
Cell and Molecular Biology Immunology in the medical area
Identifiers
URN: urn:nbn:se:oru:diva-55414DOI: 10.1111/apm.12645ISI: 000393563800012PubMedID: 28120492Scopus ID: 2-s2.0-85010471320OAI: oai:DiVA.org:oru-55414DiVA: diva2:1080611
Funder
Swedish Heart Lung FoundationKnowledge Foundation
Note

Funding Agency:

Foundation of Olle Engkvist

Available from: 2017-03-10 Created: 2017-03-10 Last updated: 2017-03-27Bibliographically approved

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Palm, EleonorDemirel, IsakBengtsson, TorbjörnKhalaf, Hazem
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