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Quantitative fucK gene polymerase chain reaction on sputum and nasopharyngeal secretions to detect Haemophilus influenzae pneumonia
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden; Department of Clinical Mycobacteriology, National Center for Diseases Control, Benghazi, Libyan Arab Jamahiriya.
Department of Infectious Diseases, Örebro University Hospital, Örebro, Sweden; Department of Infectious Diseases, Karolinska University Hospital, Stockholm, Sweden.
Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
Section of Clinical Virology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.ORCID iD: 0000-0001-6492-2491
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2013 (English)In: Diagnostic microbiology and infectious disease, ISSN 0732-8893, E-ISSN 1879-0070, Vol. 76, no 2, 141-146 p.Article in journal (Refereed) Published
Abstract [en]

A quantitative polymerase chain reaction (PCR) for the fucK gene was developed for specific detection of Haemophilus influenzae. The method was tested on sputum and nasopharyngeal aspirate (NPA) from 78 patients with community-acquired pneumonia (CAP). With a reference standard of sputum culture and/or serology against the patient's own nasopharyngeal isolate, H. influenzae etiology was detected in 20 patients. Compared with the reference standard, fucK PCR (using the detection limit 10(5) DNA copies/mL) on sputum and NPA showed a sensitivity of 95.0% (19/20) in both cases, and specificities of 87.9% (51/58) and 89.5% (52/58), respectively. In a receiver operating characteristic curve analysis, sputum fucK PCR was found to be significantly superior to sputum P6 PCR for detection of H. influenzae CAP. NPA fucK PCR was positive in 3 of 54 adult controls without respiratory symptoms. In conclusion, quantitative fucK real-time PCR provides a sensitive and specific identification of H. influenzae in respiratory secretions.

Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 76, no 2, 141-146 p.
Keyword [en]
H. influenzae, Pneumonia, Real-time PCR, fucK
National Category
Infectious Medicine Microbiology in the medical area
Identifiers
URN: urn:nbn:se:oru:diva-56356DOI: 10.1016/j.diagmicrobio.2013.02.015ISI: 000319717500005PubMedID: 23541117ScopusID: 2-s2.0-84877821138OAI: oai:DiVA.org:oru-56356DiVA: diva2:1081870
Note

Funding Agency:

Regional Research Council Uppsala-Örebro

Available from: 2017-03-15 Created: 2017-03-15 Last updated: 2017-04-07Bibliographically approved

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