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Downregulation of platelet activation markers during long-term immobilization
Dept Cardiol, Örebro Univ Hosp, Örebro, Sweden.
Örebro University Hospital. Dept Cardiol.
Dept Ecol Physiol & Ethol, Dept Ecol, Inst Pluridisciplinaire Hubert Curien, Strasbourg, France.
Dept Clin Chem, Uppsala Univ, Uppsala, Sweden.
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2013 (English)In: Platelets, ISSN 0953-7104, E-ISSN 1369-1635, Vol. 24, no 5, p. 369-374Article in journal (Refereed) Published
Abstract [en]

Immobilization and sedentary lifestyle are risk factors for venous thromboembolism and cardiovascular disease, yet little is known about platelet function during long-term physical inactivity. Our aim was to investigate platelet activation markers and their coupling to standardized immobilization: platelet-derived growth factor (PDGF-BB) and P-selectin. We studied 15 healthy females participating in the Women International Space simulation for Exploration study. Following a 20-day ambulatory control period, the subjects underwent 60 days of bed rest in head-down tilt position (-6 degrees) 24 hours a day, finalized by 20 days of recovery. The subjects were randomized into two groups during bed rest: a control group (n = 8) that remained physically inactive and an exercise group (n = 7) that participated in both supine resistance and aerobic exercise training. Blood samples for the analysis of platelet activation markers were collected at baseline (5 days before bed rest), after 44 days of bed rest and 8 days into the recovery period. Compared to baseline, the levels of P-selectin and PDGF-BB decreased after bed rest (by 55%, p = 0.01 and 73%, p < 0.03, respectively) and remained decreased in the recovery period (by 76%, p < 0.001 and 78%, p < 0.02, respectively, compared to baseline). Platelet count (baseline value for the exercise group 260 000/mu l +/- 34 000 and baseline value for the control group 210 000/mu l +/- 30 000) did not change during the bed rest study (two-way repeated measurements ANOVA, p = ns). There were no statistical differences between the physically inactive and the exercise group. During long-term immobilization, a known risk factor for thrombosis, the levels of P-selectin and PDGF-BB decreased. Our findings indicate downregulation of platelet activation during immobilization.

Place, publisher, year, edition, pages
Taylor & Francis, 2013. Vol. 24, no 5, p. 369-374
Keyword [en]
Platelets, P-selectin, PDGF, immobilization, thrombosis
National Category
Hematology Cardiac and Cardiovascular Systems
Identifiers
URN: urn:nbn:se:oru:diva-56417DOI: 10.3109/09537104.2012.715215ISI: 000321065000005PubMedID: 22931233Scopus ID: 2-s2.0-84879764932OAI: oai:DiVA.org:oru-56417DiVA, id: diva2:1082247
Available from: 2017-03-16 Created: 2017-03-16 Last updated: 2018-05-27Bibliographically approved
In thesis
1. Immobilization as a risk factor for arterial and venous thrombosis
Open this publication in new window or tab >>Immobilization as a risk factor for arterial and venous thrombosis
2018 (Swedish)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Aim: Immobilization and a sedentary lifestyle are correlated with an elevated risk of both arterial and venous thrombosis. The goal of this research was to investigate whether markers associated with cardiovascular disease risk are altered during long term immobilization in a human model and in the brown bear, which survives annual cycles of long-term immobilization.

Methods: In study populations assigned to 20-60 days of strict head-down-tilt bed rest 24h a day, we analysed blood levels of the emerging cardiovascular disease marker cystatin C, soluble markers of in vivo platelet activation P-selectin and PDGF-BB, and platelet aggregation. Blood samples were taken from free-ranging brown bears in summer and again during hibernation for analysis of lipid profile and platelet aggregation. Histological examination was performed on the left anterior descending coronary artery and aortic arches of bears harvested during the hunting season.

Results: During prolonged bed rest in humans, levels of cystatin C and platelet aggregation remained unchanged, but we observed a significant decrease in platelet activation markers. Brown bear plasma lipids were elevated during hibernation compared with the active state and cholesterol levels were generally considerably higher than normal human values. The arterial specimens showed no signs of atherosclerosis. Platelet aggregation was halved during hibernation compared to the active state.

Conclusions: Long-term immobilization has effects on several cardiovascular risk factors in both humans and bears. Increased knowledge and understanding of the protective mechanisms that allows the brown bear to survive repeated periods of immobilization could contribute to new strategies for prevention and treatment of cardiovascular disease in humans.

Place, publisher, year, edition, pages
Örebro: Örebro University, 2018. p. 69
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 179
Keyword
Venous thrombosis, arterial thrombosis, atherosclerosis, cystatin C, cholesterol, platelet activation, platelet aggregation, immobilization
National Category
General Practice
Identifiers
urn:nbn:se:oru:diva-65003 (URN)978-91-7529-243-4 (ISBN)
Public defence
2018-06-07, Örebro universitet, Campus USÖ, hörsal C3, Södra Grev Rosengatan 32, Örebro, 09:15 (Swedish)
Opponent
Supervisors
Available from: 2018-02-14 Created: 2018-02-14 Last updated: 2018-05-15Bibliographically approved

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