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Keratinocytes and Head and Neck Squamous Cell Carcinoma Cells Regulate Urokinase-type Plasminogen Activator and Plasminogen Activator Inhibitor-1 in Fibroblasts
Uppsala Univ, Dept Surg Sci, S-75185 Uppsala, Sweden..
Örebro University Hospital. Clin Res Ctr.
Univ Hosp, Clin Res Ctr, Orebro, Sweden..
Turku Univ Hosp, Dept Otorhinolaryngol Head & Neck Surg & Med Bio, FIN-20520 Turku, Finland.;Univ Turku, Turku, Finland..
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2013 (English)In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 33, no 8, 3113-3118 p.Article in journal (Refereed) Published
Abstract [en]

Background: To investigate possible differences in the effects of soluble factors from oral squamous cell carcinoma (SCC) cells (UT-SCC-87) and normal oral keratinocytes (NOK) on fibroblast expression of genes involved in tumor stroma turnover. Materials and Methods: Transwell co-cultures with fibroblasts in collagen gels, and SCC cells or NOK in inserts were carried out. Fibroblast gene expression was measured with real-time polymerase chain reaction (PCR). Results: The expression of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) was up-regulated in co-cultures with SCC cells but not with NOK. In contrast, both SCC cells and NOK regulated matrix metalloproteinase-1 (MMP1) and -3, and tissue inhibitor of metalloproteinases-2 (TIMP2) and -3 to a similar extent, while MMP2 and TIMP1 were largely unaffected. Interleukin 1 alpha (IL1 alpha) up-regulated both MMP1 and MMP3 and down-regulated PAI-1, TIMP2 and -3. Conclusion: SCC and NOK regulate fibroblast expression of genes involved in tumor stroma turnover differentially in vitro. These observations may contribute to a better understanding of the mechanisms behind extracellular matrix turnover in tumors.

Place, publisher, year, edition, pages
2013. Vol. 33, no 8, 3113-3118 p.
Keyword [en]
Head and neck cancer, keratinocytes, differential regulation, PAI-1, uPA
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:oru:diva-56550ISI: 000322559300018PubMedID: 23898067OAI: oai:DiVA.org:oru-56550DiVA: diva2:1082721
Available from: 2017-03-17 Created: 2017-03-17 Last updated: 2017-11-29Bibliographically approved

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