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Solid-phase classical complement activation by C-reactive protein (CRP) is inhibited by fluid-phase CRP-C1q interaction
Division of Rheumatology, AIR, Department of Molecular and Clinical Medicine, Linköping, Sweden.
Division of Rheumatology, AIR, Department of Molecular and Clinical Medicine, Linköping, Sweden.
Cardiovascular Inflammation Research Center, Division of Pharmacology, Department of Medicine and Care, Linköping, Sweden.
Materials in Medicine, Division of Applied Physics, Department of Physics, Chemistry and Biology, Linköping, Sweden.
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2007 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 352, no 1, 251-258 p.Article in journal (Refereed) Published
Abstract [en]

C-reactive protein (CRP) interacts with phosphorylcholine (PC), Fcgamma receptors, complement factor C1q and cell nuclear constituents, yet its biological roles are insufficiently understood. The aim was to characterize CRP-induced complement activation by ellipsometry. PC conjugated with keyhole limpet hemocyanin (PC-KLH) was immobilized to cross-linked fibrinogen. A low-CRP serum with different amounts of added CRP was exposed to the PC-surfaces. The total serum protein deposition was quantified and deposition of IgG, C1q, C3c, C4, factor H, and CRP detected with polyclonal antibodies. The binding of serum CRP to PC-KLH dose-dependently triggered activation of the classical pathway. Unexpectedly, the activation was efficiently down-regulated at CRP levels > 150 mg/L. Using radial immunodiffusion, CRP-C1q interaction was observed in serum samples with high CRP concentrations. We propose that the underlying mechanism depends on fluid-phase interaction between C1q and CRP. This might constitute another level of complement regulation, which has implications for systemic lupus erythematosus where CRP is often low despite flare-ups.

Place, publisher, year, edition, pages
Elsevier, 2007. Vol. 352, no 1, 251-258 p.
Keyword [en]
C-reactive protein; C1q; complement; inflammation; opsonization; pentraxins; systemic lupus erythematosus
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-56659DOI: 10.1016/j.bbrc.2006.11.013ISI: 000243056700041PubMedID: 17113035Scopus ID: 2-s2.0-33751433364OAI: oai:DiVA.org:oru-56659DiVA: diva2:1083500
Available from: 2017-03-21 Created: 2017-03-21 Last updated: 2017-04-12Bibliographically approved

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