Evaluation of the rapid loop-mediated isothermal amplification assay Illumigene for diagnosis of Clostridium difficile in an outbreak situation
2014 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 122, no 2, 155-160 p.Article in journal (Refereed) Published
An outbreak of Clostridium difficile infection (CDI) at Hoglandet Hospital Eksjo in southern Sweden in 2011 was mainly due to a multidrug-resistant PCR ribotype 046 (30% of all samples). Diagnostics used routinely was the Vidas CDAB assay, but to control the outbreak the rapid loop-mediated isothermal amplification (LAMP) assay Illumigene was introduced and both techniques were compared to Toxigenic culture (TC) prospectively. The LAMP assay had a superior sensitivity, that is, 98% compared to 79% for the Vidas CDAB assay. Most importantly, the mean turn-around-time from collecting sample to result was reduced from 59h to 2h enabling early isolation of patients and effective hygiene precautions. This may potentially decrease the morbidity and nosocomial transmissions of C. difficile.
Place, publisher, year, edition, pages
2014. Vol. 122, no 2, 155-160 p.
Clostridium difficile, diagnostics, Illumigene, LAMP, Vidas CDAB
Immunology in the medical area Microbiology in the medical area
IdentifiersURN: urn:nbn:se:oru:diva-56669DOI: 10.1111/apm.12121ISI: 000331081100009PubMedID: 23758095OAI: oai:DiVA.org:oru-56669DiVA: diva2:1083548