oru.sePublikationer
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model
Midwestern Univ, Chicago Coll Osteopath Med, Dept Microbiol & Immunol, Downers Grove, IL 60516 USA..
Midwestern Univ, Chicago Coll Osteopath Med, Dept Microbiol & Immunol, Downers Grove, IL 60516 USA..
Univ Southampton, Dept Clin & Expt Sci, Mol Microbiol Grp, Fac Med, Southampton, Hants, England..
Univ Southampton, Dept Clin & Expt Sci, Mol Microbiol Grp, Fac Med, Southampton, Hants, England..ORCID iD: 0000-0002-4938-1620
Show others and affiliations
2014 (English)In: Pathogens and Disease, E-ISSN 2049-632X, Vol. 70, no 1, 61-69 p.Article in journal (Refereed) Published
Abstract [en]

We hypothesized that the plasmid of urogenital isolates of Chlamydia trachomatis would modulate infectivity and virulence in a mouse model. To test this hypothesis, we infected female mice in the respiratory or urogenital tract with graded doses of a human urogenital isolate of C.trachomatis, serovar F, possessing the cognate plasmid. For comparison, we inoculated mice with a plasmid-free serovar F isolate. Following urogenital inoculation, the plasmid-free isolate displayed significantly reduced infectivity compared with the wild-type strain with the latter yielding a 17-fold lower infectious dose to yield 50% infection. When inoculated via the respiratory tract, the plasmid-free isolate exhibited reduced infectivity and virulence (as measured by weight change) when compared to the wild-type isolate. Further, differences in infectivity, but not in virulence were observed in a C.trachomatis, serovar E isolate with a deletion within the plasmid coding sequence 1 when compared to a serovar E isolate with no mutations in the plasmid. We conclude that plasmid loss reduces virulence and infectivity in this mouse model. These findings further support a role for the chlamydial plasmid in infectivity and virulence in vivo.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2014. Vol. 70, no 1, 61-69 p.
Keyword [en]
Chlamydia, plasmid, mouse, virulence, infection, genome
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:oru:diva-56746DOI: 10.1111/2049-632X.12086ISI: 000331213000007PubMedID: 24022847OAI: oai:DiVA.org:oru-56746DiVA: diva2:1083965
Available from: 2017-03-23 Created: 2017-03-23 Last updated: 2017-03-23Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Clarke, Ian N.Unemo, Magnus
By organisation
Orebro University Hospital
In the same journal
Pathogens and Disease
Infectious Medicine

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 7 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf