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A study of serum biomarkers associated with relapse of cervical cancer
Orebro University Hospital.
Orebro University Hospital.
Department of Oncology, Örebro University Hospital, Örebro, Sweden.
2012 (English)In: Anticancer Research, ISSN 0250-7005, E-ISSN 1791-7530, Vol. 32, no 11, 4913-22 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND/AIM: To discover candidate protein biomarkers in the serum of patients with cervical cancer that differentiate between patients with relapse from those who are tumor-free after primary treatment with (platinum-based chemo-) radiation.

PATIENTS AND METHODS: Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) with cation exchange (CM10) and hydrophobic/reverse-phase (H50) was used to examine 44 serum samples from patients with advanced cervical cancer, primarily treated with (platinum-based chemo-) radiation.

RESULTS: Ten candidate biomarkers were identified in the serum of 34 patients. Six candidate markers were elevated in patients with no relapse and four were elevated in patients with relapse [p=0.007-0.11; area under the curve (AUC)=0.70-0.75]. Masses of candidate biomarkers ranged from 2,022 to 116,165 Da.

CONCLUSION: Patients with relapse from primary advanced cervical cancer exhibit different serum protein expression profiles from those with no relapse.

Place, publisher, year, edition, pages
2012. Vol. 32, no 11, 4913-22 p.
National Category
Cancer and Oncology
Identifiers
URN: urn:nbn:se:oru:diva-56898ISI: 000311524500037PubMedID: 23155260ScopusID: 2-s2.0-84872659639OAI: oai:DiVA.org:oru-56898DiVA: diva2:1085569
Available from: 2017-03-29 Created: 2017-03-29 Last updated: 2017-03-29Bibliographically approved
In thesis
1. Predictive and prognostic factors in cervical carcinomas treated with (chemo-) radiotherapy
Open this publication in new window or tab >>Predictive and prognostic factors in cervical carcinomas treated with (chemo-) radiotherapy
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

A series of 131 women with cervical carcinoma FIGO stage I-IV were treated with external radiotherapy and intracavitary brachytherapy. In 47 patients (36%) concomitant chemotherapy was given. One hundred and twenty-one tumors (92%) achieved complete remission. Addition of chemotherapy increased primary cure rate to 98%. Tumor stage, tumor size, and histology were significant predictive factors for primary cure. Treatment related factors were: brachytherapy dose and interruption of irradiation.

Thirty-nine recurrences (30%) were recorded. Tumor stage, histology, and concomitant chemotherapy were significant predictive factors.

The 5-year cancer-specific survival rate of the complete series was 65%. Tumor size was a strong prognostic factor in multivariate analysis.

Serum samples from 44 patients were analyzed. Ten candidate biomarker proteins with regard to tumor recurrences were identified.

Five Hedgehog proteins were analyzed with immunohistochemistry. Residual tumor, local and distant recurrences and survival rate were associated with PTCH, SMO and GLI2. In the Wnt-β-catenin study intense staining of the membranes and nuclear staining > 5% were of significant predictive and prognostic value. Intense nuclear APC staining was associated with recurrences and cancer-specific survival rate.

Conclusion: Histology, tumor size and brachytherapy dose were important clinical predictive and prognostic factors. Multiprotein analysis identified ten biomarker proteins associated with tumor recurrences. Three proteins (PTCH, SMO, and GLI2) in the Hedgehog pathway were of predictive and prognostic value. In the Wnt-β-catenin pathway intensity of β-catenin membrane staining and accumulation in the nuclei as well as nuclear APC-expression were of predictive and prognostic value.

Place, publisher, year, edition, pages
Örebro: Örebro University, 2017. 61 p.
Series
Örebro Studies in Medicine, ISSN 1652-4063 ; 159
Keyword
cervical carcinoma, radio-chemotherapy, biomarkers, predictive factors, prognostic factors, multiprotein analysis, Hedgehog pathway, Wnt/β-catenin pathway
National Category
Surgery
Research subject
Surgery
Identifiers
urn:nbn:se:oru:diva-56127 (URN)978-91-7529-185-7 (ISBN)
Public defence
2017-04-21, Örebro universitet, Campus USÖ, hörsal C1, Södra Grev Rosengatan 32, Örebro, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2017-03-06 Created: 2017-03-06 Last updated: 2017-04-19Bibliographically approved

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