oru.sePublikationer
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Genomic relatedness of Staphylococcus pettenkoferi isolates of different origins
Örebro University, School of Medical Sciences. Centre for Clinical Research, Hospital of Västmanland Västerås, Västerås, Sweden.ORCID iD: 0000-0003-2867-1044
Örebro University, School of Medical Sciences. Orebro University Hospital. Department of Laboratory Medicine.
Statens Serum Institut, Copenhagen, Denmark.
Statens Serum Institut, Copenhagen, Denmark.
Show others and affiliations
2017 (English)In: Journal of Medical Microbiology, ISSN 0022-2615, E-ISSN 1473-5644, Vol. 66, no 5, 601-608 p.Article in journal (Refereed) Published
Abstract [en]

Purpose: The aim of the study was to characterize clinical and environmental Staphylococcus pettenkoferi isolates with regard to genomic diversity and antibiotic susceptibility pattern. Repetitive-sequence-based PCR and core genome phylogenetic analysis of whole-genome sequencing (WGS) data verified the presence of distinct clades comprising closely related S. pettenkoferi isolates from different geographical locations and origins.

Methodology: Phylogenetic relationships between 25 S. pettenkoferi isolates collected from blood cultures and intra-operative air sampling were determined by repetitive-sequence-based PCR typing and analysis of similar to 157 000 SNPs identified in the core genome after WGS. Antibiotic susceptibility testing and tests for biofilm production (microtitre plate assay) were performed.

Results: Repetitive-sequence-based PCR as well as WGS data demonstrated the close relatedness of clinically significant blood culture isolates to probable contaminants, as well as to environmental isolates. Antibiotic-susceptibility testing demonstrated a low level of antimicrobial resistance. The mecA gene was present in two cefoxitin-resistant isolates. No isolates were found to produce biofilm.

Conclusion: Close genomic relatedness of S. pettenkoferi isolates from different geographical locations and origins were found within clades, but with substantial genomic difference between the two major clades. The ecological niche of S. pettenkoferi remains unconfirmed, but the presence of S. pettenkoferi in the air of the operating field favours the suggestion of a role in skin flora. Identification of S. pettenkoferi in clinical samples should, in a majority of cases, most likely be regarded as a probable contamination, and its role as a possible pathogen in immunocompromised hosts remains to be clarified.

Place, publisher, year, edition, pages
Microbiology Society , 2017. Vol. 66, no 5, 601-608 p.
Keyword [en]
Staphylococcus pettenkoferi, genotypic relatedness, repetitive-sequence based PCR typing, whole-genome sequencing
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:oru:diva-58000DOI: 10.1099/jmm.0.000472ISI: 000401984900007PubMedID: 28530888ScopusID: 2-s2.0-85019900525OAI: oai:DiVA.org:oru-58000DiVA: diva2:1108811
Note

Funding Agencies:

Örebro County Council Research Committee, Örebro, Sweden, Centre for Clinical Research, Västerås  

County Council of Västmanland Research Fund 

Available from: 2017-06-13 Created: 2017-06-13 Last updated: 2017-06-13Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Månsson, EmeliHellmark, BengtSundqvist, MartinSöderquist, Bo
By organisation
School of Medical SciencesOrebro University Hospital
In the same journal
Journal of Medical Microbiology
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 6 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf