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Transcriptional Alterations of Virulence-Associated Genes in Extended Spectrum Beta-Lactamase (ESBL)-Producing Uropathogenic Escherichia coli during Morphologic Transitions Induced by Ineffective Antibiotics
Örebro University, School of Medical Sciences. Inflammatory Response and Infection Susceptibility Centre.
Örebro University, School of Medical Sciences. (Nutrition-Gut-Brain Interactions Research Centre)ORCID iD: 0000-0003-3383-9219
School of Medical Sciences, Örebro University, Örebro, Sweden.
Örebro University, School of Medical Sciences. Faculty of Medicine and Health, Inflammatory Response and Infection Susceptibility Centre, Örebro University, Örebro, Sweden.
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2017 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 8, 1058Article in journal (Refereed) Published
Abstract [en]

It is known that an ineffective antibiotic treatment can induce morphological shifts in uropathogenic Escherichia coli (UPEC) but the virulence properties during these shifts remain to be studied. The present study examines changes in global gene expression patterns and in virulence factor-associated genes in an extended spectrum beta-lactamase (ESBL)-producing UPEC (ESBL019) during the morphologic transitions induced by an ineffective antibiotic and in the presence of human primary bladder epithelial cells. Microarray results showed that the different morphological states of ESBL019 had significant transcriptional alterations of a large number of genes (Transition; 7%, Filamentation; 32%, and Reverted 19% of the entities on the array). All three morphological states of ESBL019 were associated with a decreased energy metabolism, altered iron acquisition systems and altered adhesion expression. In addition, genes associated with LPS synthesis and bacterial motility was also altered in all the morphological states. Furthermore, the transition state induced a significantly higher release of TNF-alpha from bladder epithelial cells compared to all other morphologies, while the reverted state was unable to induce INF-alpha release. Our findings show that the morphological shifts induced by ineffective antibiotics are associated with significant transcriptional virulence alterations in ESBL-producing UPEC, which may affect survival and persistence in the urinary tract.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2017. Vol. 8, 1058
Keyword [en]
filamentation, extended-spectrum beta-lactamase, uropathogenic Escherichia coli, ineffective antibiotics, morphological plasticity
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:oru:diva-58778DOI: 10.3389/fmicb.2017.01058ISI: 000403139300001PubMedID: 28659883Scopus ID: 2-s2.0-85020493714OAI: oai:DiVA.org:oru-58778DiVA: diva2:1128575
Funder
Swedish Society of Medicine
Note

Funding Agencies:

Research Committee of Örebro County Council, Nyckelfonden

Faculty of Medicine and Health at Örebro University

Available from: 2017-07-26 Created: 2017-07-26 Last updated: 2017-10-16Bibliographically approved

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