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Prediction of non-alcoholic fatty-liver disease and liver fat content by serum molecular lipids
Örebro University, School of Medical Sciences. VTT Technical Research Centre of Finland, Tietotie 2, Espoo 02044 VTT, Finland.ORCID iD: 0000-0002-2856-9165
VTT Technical Research Centre of Finland, Tietotie 2, Espoo 02044 VTT, Finland.
Department of Medicine, Division of Diabetes, University of Helsinki, Helsinki, Finland.
VTT Technical Research Centre of Finland, Tietotie 2, Espoo 02044 VTT, Finland.
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2013 (English)In: Diabetologia, ISSN 0012-186X, E-ISSN 1432-0428, Vol. 56, no 10, 2266-74 p.Article in journal (Refereed) Published
Abstract [en]

AIMS/HYPOTHESIS: We examined whether analysis of lipids by ultra-performance liquid chromatography (UPLC) coupled to MS allows the development of a laboratory test for non-alcoholic fatty-liver disease (NAFLD), and how a lipid-profile biomarker compares with the prediction of NAFLD and liver-fat content based on routinely available clinical and laboratory data.

METHODS: We analysed the concentrations of molecular lipids by UPLC-MS in blood samples of 679 well-characterised individuals in whom liver-fat content was measured using proton magnetic resonance spectroscopy ((1)H-MRS) or liver biopsy. The participants were divided into biomarker-discovery (n = 287) and validation (n = 392) groups to build and validate the diagnostic models, respectively.

RESULTS: Individuals with NAFLD had increased triacylglycerols with low carbon number and double-bond content while lysophosphatidylcholines and ether phospholipids were diminished in those with NAFLD. A serum-lipid signature comprising three molecular lipids ('lipid triplet') was developed to estimate the percentage of liver fat. It had a sensitivity of 69.1% and specificity of 73.8% when applied for diagnosis of NAFLD in the validation series. The usefulness of the lipid triplet was demonstrated in a weight-loss intervention study.

CONCLUSIONS/INTERPRETATION: The liver-fat-biomarker signature based on molecular lipids may provide a non-invasive tool to diagnose NAFLD, in addition to highlighting lipid molecular pathways involved in the disease.

Place, publisher, year, edition, pages
Springer, 2013. Vol. 56, no 10, 2266-74 p.
Keyword [en]
Lipidomics; Mass spectrometry; Non-alcoholic fatty-liver disease
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:oru:diva-59356DOI: 10.1007/s00125-013-2981-2ISI: 000324062800021PubMedID: 23824212Scopus ID: 2-s2.0-84890116926OAI: oai:DiVA.org:oru-59356DiVA: diva2:1135891
Note

Funding Agencies:

Academy of Finland  

Sigrid Juselius Foundation  

Finnish Diabetes Research Foundation  

Emil Aaltonen Foundation  

Finnish Medical Foundation  

Novo Nordisk Foundation  

Academy of Finland Centre of Excellence in Molecular Systems Immunology and Physiology Research - SyMMyS  250114 

European Commission  LSHM-CT-2005-018734 

Innovative Medicines Initiative Joint Undertaking  115372 

European Union  

EFPIA companies 

Available from: 2017-08-24 Created: 2017-08-24 Last updated: 2017-09-06Bibliographically approved

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