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Metabolic transformations of dietary polyphenols: comparison between in vitro colonic and hepatic models and in vivo urinary metabolites
Department of Clinical Medicine and Surgery, “Federico II” University, Naples, Italy.
Department of Clinical Medicine and Surgery, “Federico II” University, Naples, Italy.
Department of Clinical Medicine and Surgery, “Federico II” University, Naples, Italy.
Sahlgrenska Center for Cardiovascular and Metabolic Research/Wallenberg Laboratory, Department of Molecular and Clinical Medicine, University of Gothenburg, Gothenburg, Sweden; Department of Mathematical Sciences, University of Gothenburg, Gothenburg, Sweden.
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2016 (English)In: Journal of Nutritional Biochemistry, ISSN 0955-2863, E-ISSN 1873-4847, Vol. 33, p. 111-118Article in journal (Refereed) Published
Abstract [en]

Studies on metabolism of polyphenols have revealed extensive transformations in the carbon backbone by colonic microbiota; however, the influence of microbial and hepatic transformations on human urinary metabolites has not been explored. Therefore, the aims of this study were (1) to compare the in vitro microbial phenolic metabolite profile of foods and beverages with that excreted in urine of subjects consuming the same foodstuff and (2) to explore the role of liver on postcolonic metabolism of polyphenols by using in vitro hepatic models. A 24-h urinary phenolic metabolite profile was evaluated in 72 subjects participating in an 8-week clinical trial during which they were randomly assigned to diets differing for polyphenol content. Polyphenol-rich foods and beverages used in the clinical trial were subjected to human fecal microbiota in the in vitro colon model. Metabolites from green tea, one of the main components of the polyphenol-rich diet, were incubated with primary hepatocytes to highlight hepatic conversion of polyphenols. The analyses were performed using targeted gas chromatography with mass spectrometer (GCxGC-TOFMS:colon model; GC-MS: urine and hepatocytes). A significant correlation was found between urinary and colonic metabolites with C1-C3 side chain (P=.040). However, considerably higher amounts of hippuric acid, 3-hydroxybenzoic acid and ferulic acid were detected in urine than in the colon model. The hepatic conversion showed additional amounts of these metabolites complementing the gap between in vitro colon model and the in vivo urinary excretion. Therefore, combining in vitro colon and hepatic models may better elucidate the metabolism of polyphenols from dietary exposure to urinary metabolites.

Place, publisher, year, edition, pages
New York, USA: Elsevier, 2016. Vol. 33, p. 111-118
Keyword [en]
In vitro colon model, Metabolism, Phenolic carbon backbone, Polyphenols, Primary hepatocytes
National Category
Medical and Health Sciences Gastroenterology and Hepatology
Identifiers
URN: urn:nbn:se:oru:diva-59375DOI: 10.1016/j.jnutbio.2016.03.007ISI: 000378011400013PubMedID: 27155917Scopus ID: 2-s2.0-84964988371OAI: oai:DiVA.org:oru-59375DiVA, id: diva2:1136144
Note

Funding Agencies:

ETHERPATHS project (European Commission), Projektnr. FP7-KBBE-222,639

Ministero dell'Istruzione, dell'Universita della Ricerca, Rome, Italy (PRIN), Projektnr. 2010JC WWKM

Available from: 2017-08-25 Created: 2017-08-25 Last updated: 2018-01-30Bibliographically approved

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Oresic, Matej

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