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Dual action of bacteriocin PLNC8 alpha beta through inhibition of Porphyromonas gingivalis infection and promotion of cell proliferation
Örebro University, School of Medical Sciences.
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Örebro University, School of Medical Sciences. Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.
School of Medical Sciences, Örebro University, Örebro, Sweden.
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2017 (English)In: Pathogens and Disease, E-ISSN 2049-632X, Vol. 75, no 5, ftx064Article in journal (Refereed) Published
Abstract [en]

Periodontitis is a chronic inflammatory disease that is characterised by accumulation of pathogenic bacteria, including Porphyromonas gingivalis, in periodontal pockets. The lack of effective treatments has emphasised in an intense search for alternative methods to prevent bacterial colonisation and disease progression. Bacteriocins are bacterially produced antimicrobial peptides gaining increased consideration as alternatives to traditional antibiotics. We show rapid permeabilisation and aggregation of P. gingivalis by the two-peptide bacteriocin PLNC8 alpha beta. In a cell culture model, P. gingivalis was cytotoxic against gingival fibroblasts. The proteome profile of fibroblasts is severely affected by P. gingivalis, including induction of the ubiquitin-proteasome pathway. PLNC8 alpha beta enhanced the expression of growth factors and promoted cell proliferation, and suppressed proteins associated with apoptosis. PLNC8 alpha beta efficiently counteracted P. gingivalis-mediated cytotoxicity, increased expression of a large number of proteins and restored the levels of inflammatory mediators. In conclusion, we show that bacteriocin PLNC8 alpha beta displays dual effects by acting as a potent antimicrobial agent killing P. gingivalis and as a stimulatory factor promoting cell proliferation. We suggest preventive and therapeutical applications of PLNC8 alpha beta in periodontitis to supplement the host immune defence against P. gingivalis infection and support wound healing processes.

Place, publisher, year, edition, pages
Oxford University Press, 2017. Vol. 75, no 5, ftx064
Keyword [en]
Porphyromonas gingivalis, periodontitis, cell proliferation, proteomics, bacteriocin, PLNC8
National Category
Immunology in the medical area Infectious Medicine Microbiology in the medical area
Identifiers
URN: urn:nbn:se:oru:diva-59311DOI: 10.1093/femspd/ftx064ISI: 000407245300014OAI: oai:DiVA.org:oru-59311DiVA: diva2:1136155
Funder
Knowledge Foundation, 20150244 20150086
Note

Funding Agency:

Foundation of Magnus Bergvall  2015-00823

Available from: 2017-08-25 Created: 2017-08-25 Last updated: 2017-08-25Bibliographically approved

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