To Örebro University

oru.seÖrebro University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Dual action of bacteriocin PLNC8 alpha beta through inhibition of Porphyromonas gingivalis infection and promotion of cell proliferation
Örebro University, School of Medical Sciences.
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Örebro University, School of Medical Sciences. Division of Molecular Physics, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.
School of Medical Sciences, Örebro University, Örebro, Sweden.
Show others and affiliations
2017 (English)In: Pathogens and Disease, E-ISSN 2049-632X, Vol. 75, no 5, article id ftx064Article in journal (Refereed) Published
Abstract [en]

Periodontitis is a chronic inflammatory disease that is characterised by accumulation of pathogenic bacteria, including Porphyromonas gingivalis, in periodontal pockets. The lack of effective treatments has emphasised in an intense search for alternative methods to prevent bacterial colonisation and disease progression. Bacteriocins are bacterially produced antimicrobial peptides gaining increased consideration as alternatives to traditional antibiotics. We show rapid permeabilisation and aggregation of P. gingivalis by the two-peptide bacteriocin PLNC8 alpha beta. In a cell culture model, P. gingivalis was cytotoxic against gingival fibroblasts. The proteome profile of fibroblasts is severely affected by P. gingivalis, including induction of the ubiquitin-proteasome pathway. PLNC8 alpha beta enhanced the expression of growth factors and promoted cell proliferation, and suppressed proteins associated with apoptosis. PLNC8 alpha beta efficiently counteracted P. gingivalis-mediated cytotoxicity, increased expression of a large number of proteins and restored the levels of inflammatory mediators. In conclusion, we show that bacteriocin PLNC8 alpha beta displays dual effects by acting as a potent antimicrobial agent killing P. gingivalis and as a stimulatory factor promoting cell proliferation. We suggest preventive and therapeutical applications of PLNC8 alpha beta in periodontitis to supplement the host immune defence against P. gingivalis infection and support wound healing processes.

Place, publisher, year, edition, pages
Oxford University Press, 2017. Vol. 75, no 5, article id ftx064
Keywords [en]
Porphyromonas gingivalis, periodontitis, cell proliferation, proteomics, bacteriocin, PLNC8
National Category
Immunology in the medical area Infectious Medicine Microbiology in the medical area
Identifiers
URN: urn:nbn:se:oru:diva-59311DOI: 10.1093/femspd/ftx064ISI: 000407245300014Scopus ID: 2-s2.0-85028656048OAI: oai:DiVA.org:oru-59311DiVA, id: diva2:1136155
Funder
Knowledge Foundation, 20150244 20150086
Note

Funding Agency:

Foundation of Magnus Bergvall 2015-00823  

Available from: 2017-08-25 Created: 2017-08-25 Last updated: 2024-01-02Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus

Authority records

Bengtsson, TorbjörnSelegård, RobertKhalaf, Hazem

Search in DiVA

By author/editor
Bengtsson, TorbjörnSelegård, RobertKhalaf, Hazem
By organisation
School of Medical Sciences
In the same journal
Pathogens and Disease
Immunology in the medical areaInfectious MedicineMicrobiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 688 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf