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Randomized clinical trial: Effective gluten degradation by Aspergillus niger-derived enzyme in a complex meal setting
Örebro University, School of Medical Sciences. (Nutrition-Gut-Brain Interactions Research Centre (NGBI))ORCID iD: 0000-0003-0466-1861
Örebro University, School of Medical Sciences. (Nutrition-Gut-Brain Interactions Research Centre (NGBI))ORCID iD: 0000-0002-4204-6811
DSM Biotechnology Centre, Delft, Netherlands.
Örebro University, School of Medical Sciences. (Nutrition-Gut-Brain Interactions Research Centre (NGBI))ORCID iD: 0000-0002-0362-0008
2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, no 13100Article in journal (Refereed) Published
Abstract [en]

The Aspergillus niger-derived prolyl endoprotease (AN-PEP) has previously been shown to degrade gluten in healthy subjects when added to an intragastrically infused meal. The current study investigated the efficacy of AN-PEP in a physiological meal setting. In this randomized placebo-controlled crossover study, 18 gluten-sensitive subjects consumed a porridge containing 0.5 g gluten together with two tablets either containing a high or low dose of AN-PEP, or placebo. Gastric and duodenal content was sampled over 180 minutes, and areas under the curve of gluten concentrations were calculated. The primary outcome, i.e. success rate of high dose AN-PEP defined as at least 50% gluten degradation compared to placebo in the duodenum, was achieved in 10 of 13 comparisons. In the stomach, gluten levels were reduced from 176.9 (median, interquartile range 73.5–357.8) to 22.0 (10.6–50.8, p = 0.001) in the high dose and to 25.4 μg × min/ml (16.4–43.7, p = 0.001) in the low dose. In the duodenum, gluten levels were reduced from 14.1 (8.3–124.7) in the placebo to 6.3 (3.5–19.8, p = 0.019) in the high dose and to 7.4 μg × min/ml in the low dose (3.8–12.0, p = 0.015). Thus even in a physiological meal setting, AN-PEP significantly degraded most gluten in the stomach before it entered the duodenum.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017. Vol. 7, no 13100
Keywords [en]
Gluten
National Category
Nutrition and Dietetics
Research subject
Nutrition
Identifiers
URN: urn:nbn:se:oru:diva-61493DOI: 10.1038/s41598-017-13587-7ISI: 000412950900035PubMedID: 29026170Scopus ID: 2-s2.0-85031294461OAI: oai:DiVA.org:oru-61493DiVA, id: diva2:1148724
Note

Funding Agency:

DSM

Available from: 2017-10-12 Created: 2017-10-12 Last updated: 2017-11-10Bibliographically approved

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König, JuliaHolster, SavanneBrummer, Robert Jan

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