oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Signal Enhancement in the HPLC-ESI-MS/MS analysis of spironolactone and its metabolites using HFIP and NH4F as eluent additives
Institute of Chemistry, University of Tartu, Tartu, Estonia.
Institute of Chemistry, University of Tartu, Tartu, Estonia.ORCID iD: 0000-0002-5360-2326
Children’s Clinic, Tartu University Hospital, Tartu, Estonia; Institute of Biomedicine and Translational Medicine, Department of Pharmacology, University of Tartu, Tartu, Estonia.
Children’s Clinic, Tartu University Hospital, Tartu, Estonia.
Show others and affiliations
2017 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 409, no 12, p. 3145-3151Article in journal (Refereed) Published
Abstract [en]

This paper describes an LC-MS/MS method to determine the concentration of spironolactone and its metabolites 7-alpha-methylthiospironolactone and canrenone in blood plasma samples. The resulting assay is simple (using protein precipitation for sample preparation) and sensitive (the lower limit of quantification is close to 0.5 ng/ml) while requiring only 50 mu l of plasma, making it especially suitable for analyzing samples obtained from pediatric and neonatal patients where sample sizes are limited. The sensitivity is achieved by using ammonium fluoride as an eluent additive, which in our case amplifies the signal from our analytes in the plasma solution on average about 70 times. The method is fully validated according to the European Medicines Agency's guideline and used for the measurement of pediatric patients' samples in clinical trials for evaluating oral spironolactone's and its metabolites' pharmacokinetics in children up to 2 years of age.

Place, publisher, year, edition, pages
Springer, 2017. Vol. 409, no 12, p. 3145-3151
Keywords [en]
Spironolactone; Ammonium fluoride; Signal enhancement; Liquid chromatography-mass spectrometry; Ionization efficiency; Hexafluoroisopropanol
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
Identifiers
URN: urn:nbn:se:oru:diva-61888DOI: 10.1007/s00216-017-0255-4ISI: 000399700700009PubMedID: 28224249Scopus ID: 2-s2.0-85013466865OAI: oai:DiVA.org:oru-61888DiVA, id: diva2:1150601
Note

Funding Agencies:

ERANet PRIOMEDCHILD program by the Estonian Science Foundation  18/2011 

People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7)  608765 

PUTJD 22 from the Estonian Research Council  

Ministry of Education and Research of Estonia  IUT20-14 (TLOKT14014I) 

Available from: 2017-10-19 Created: 2017-10-19 Last updated: 2018-08-07Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Aro, Rudolf

Search in DiVA

By author/editor
Aro, Rudolf
In the same journal
Analytical and Bioanalytical Chemistry
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 235 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf