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Expression of HLA-DRA and CD74 mRNA in whole blood during the course of complicated and uncomplicated Staphylococcus aureus bacteremia
Örebro University, School of Medical Sciences. Department of Infectious Diseases, Örebro University Hospital, Örebro University, Örebro, Sweden; Faculty of Medicine and Health, School of Medical Sciences, Örebro University, Örebro, Sweden.
Örebro University, School of Medical Sciences. Örebro University Hospital. Department of Infectious Diseases.
Örebro University, School of Medical Sciences. Örebro University Hospital. Department of Clinical Research Laboratory.
Department of Infectious Diseases, Örebro University Hospital, Örebro University, Örebro, Sweden; Faculty of Medicine and Health, School of Medical Sciences, Örebro University, Örebro, Sweden.ORCID iD: 0000-0003-2625-4597
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2017 (English)In: Microbiology and immunology, ISSN 0385-5600, E-ISSN 1348-0421, Vol. 61, no 10, p. 442-451Article in journal (Refereed) Published
Abstract [en]

To improve management of Staphylococcus aureus bacteremia (SAB), better understanding of host-pathogen interactions is needed. In vitro studies have shown that S. aureus bacteria induce dose-dependent immunosuppression that is evidenced by reduced expression of major histocompatibility complex (MHC) class II on antigen presenting cells. Thus, the aim of this study was to determine whether expression of the MHC class II-related genes HLA-DRA and CD74 is more greatly reduced in complicated SAB, with its probable higher loads of S. aureus, than in uncomplicated SAB. Adult patients with SAB were prospectively included and blood samples taken on the day of confirmation of SAB (Day 1) and on Days 2, 3, 5 and 7. HLA-DRA and CD74 mRNA expression was determined by quantitative reverse transcription PCR. Sepsis was defined according to the Sepsis-3 classification and SAB was categorized as complicated in patients with deep-seated infection and/or hematogenous seeding. Twenty patients with SAB were enrolled and samples obtained on all assessment days. HLA-DRA and CD74 expression did not differ significantly between patients with SAB and sepsis (n=13) and those without sepsis (n=7) on any assessment day. However, patients with complicated SAB (n=14) had significantly weaker HLA-DRA expression on all five assessment days than patients with uncomplicated SAB (n=6). Additionally, they tended to have weaker CD74 expressions. Neutrophil, monocyte and leukocyte counts did not differ significantly between complicated and uncomplicated SAB. In conclusion, patients with complicated SAB show weaker HLA-DRA expression than those with uncomplicated SAB during the first week of bacteremia.

Place, publisher, year, edition, pages
Wiley-Blackwell Publishing Asia , 2017. Vol. 61, no 10, p. 442-451
Keyword [en]
CD74, HLA-DRA, sepsis, Staphylococcus aureus
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:oru:diva-62068DOI: 10.1111/1348-0421.12533ISI: 000412860400005PubMedID: 28862321Scopus ID: 2-s2.0-85032877412OAI: oai:DiVA.org:oru-62068DiVA, id: diva2:1153386
Note

Funding Agency:

Research Committee of Örebro County Council

Available from: 2017-10-30 Created: 2017-10-30 Last updated: 2018-02-23Bibliographically approved

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Rasmussen, GunlögCajander, SaraBäckman, AndersKällman, JanSöderquist, Bo

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