oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Galectin-3 alters the lateral mobility and clustering of β1-integrin receptors
Alberta Glycomics Centre, Department of Chemistry, University of Alberta, Edmonton, AB, Canada.
Örebro University, School of Medical Sciences. Alberta Glycomics Centre, Department of Chemistry, University of Alberta, Edmonton, AB, Canada.
Alberta Glycomics Centre, Department of Chemistry, University of Alberta, Edmonton, AB, Canada.
Alberta Glycomics Centre, Department of Chemistry, University of Alberta, Edmonton, AB, Canada; Ludwig-Maximilians-University, Munich, Germany.
Show others and affiliations
2017 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 10, article id e0184378Article in journal (Refereed) Published
Abstract [en]

Glycoprotein receptors are influenced by myriad intermolecular interactions at the cell surface. Specific glycan structures may interact with endogenous lectins that enforce or disrupt receptor-receptor interactions. Glycoproteins bound by multivalent lectins may form extended oligomers or lattices, altering the lateral mobility of the receptor and influencing its function through endocytosis or changes in activation. In this study, we have examined the interaction of Galectin-3 (Gal-3), a human lectin, with adhesion receptors. We measured the effect of recombinant Gal-3 added exogenously on the lateral mobility of the alpha 5 beta 1 integrin on HeLa cells. Using single-particle tracking (SPT) we detected increased lateral mobility of the integrin in the presence of Gal-3, while its truncated C-terminal domain (Gal-3C) showed only minor reductions in lateral mobility. Treatment of cells with Gal-3 increased beta 1-integrin mediated migration with no apparent changes in viability. In contrast, Gal-3C decreased both cell migration and viability. Fluorescence microscopy allowed us to confirm that exogenous Gal-3 resulted in reorganization of the integrin into larger clusters. We used a proteomics analysis to confirm that cells expressed endogenous Gal-3, and found that addition of competitive oligosaccharide ligands for the lectin altered the lateral mobility of the integrin. Together, our results are consistent with a Gal-3-integrin lattice model of binding and confirm that the lateral mobility of integrins is natively regulated, in part, by galectins.

Place, publisher, year, edition, pages
Public Library of Science , 2017. Vol. 12, no 10, article id e0184378
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:oru:diva-62163DOI: 10.1371/journal.pone.0184378ISI: 000412627400008PubMedID: 29016609Scopus ID: 2-s2.0-85031047269OAI: oai:DiVA.org:oru-62163DiVA, id: diva2:1155246
Note

Funding Agencies:

Alberta Glycomics Centre  

Natural Sciences and Engineering Research Council of Canada (NSERC)  

NSERC CGS-D scholarship  

University of Alberta Research Experience (UARE) program 

Available from: 2017-11-07 Created: 2017-11-07 Last updated: 2017-11-29Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Rode, Julia

Search in DiVA

By author/editor
Rode, Julia
By organisation
School of Medical Sciences
In the same journal
PLoS ONE
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 55 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf