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Harmonizing Lipidomics: NIST Interlaboratory Comparison Exercise for Lipidomics using Standard Reference Material 1950 Metabolites in Frozen Human Plasma
Marine Biochemical Sciences Group, Chemical Sciences Division, Hollings Marine Laboratory, National Institute of Standards and Technology, Charleston SC, USA.
Örebro University, School of Science and Technology.
Örebro University, School of Medical Sciences. Hollings Marine Laboratory, Marine Biochemical Sciences Group, National Institute of Standards and Technology, Charleston SC, United States; Division of Laboratory Sciences, Centers for Disease Control and Prevention, Atlanta GA, United States.ORCID iD: 0000-0002-2856-9165
Department of Chemistry and Biochemistry, Wayne State University, Detroit MI, USA.
Number of Authors: 94
2017 (English)In: Journal of Lipid Research, ISSN 0022-2275, E-ISSN 1539-7262, Vol. 58, no 12, p. 2275-2288Article in journal (Refereed) Published
Abstract [en]

As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950 Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each lab using a different lipidomics workflow. A total of 1527 unique lipids were measured across all laboratories, and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra- and inter-laboratory quality control and method validation. These analyses were performed using non-standardized laboratory-independent workflows. The consensus locations were also compared to a previous examination of SRM 1950 by the LIPID MAPS consortium. While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.

Place, publisher, year, edition, pages
American Society for Biochemistry and Molecular Biology, 2017. Vol. 58, no 12, p. 2275-2288
Keyword [en]
Fatty Acyls, Glycerolipids, Lipidomics, Lipids, Phospholipids, Sphingolipids, Standard Reference Material 1950, Sterols, interlaboratory comparison exercise, quantitation
National Category
Clinical Laboratory Medicine Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-62436DOI: 10.1194/jlr.M079012ISI: 000416963900004PubMedID: 28986437Scopus ID: 2-s2.0-85037028318OAI: oai:DiVA.org:oru-62436DiVA: diva2:1163430
Funder
Swedish Research Council, 2015-4870Swedish Heart Lung Foundation, HLF 20140469 HLF 20150640
Note

Funding Agencies:

University of Victoria U24 DK097209, UL1 TL001873

Kyushu University

Life Sciences Institute

Austrian Science Fund P26148-N19

National Research Foundation Singapore NRFI2015-05

Genome British Columbia 7203

Genome British Columbia MC3T

Genome British Columbia P01 HL034300

Genome British Columbia Spectrometry Core

Genome British Columbia 205MET

Genome British Columbia 215MET

McGill University DBI-1228622 P20-RR16475 MCB-0920663 MCB-413036 DBI-0521587 EPS-0236913

Natural Sciences and Engineering Research Council of Canada

Diabetes Research and Training Center

Japan Science and Technology Agency JPMJCR1395

McGill University

National University of Singapore

Advanced Low Carbon Technology Research and Development Program

Albert Einstein College of Medicine, Yeshiva University

Leading Edge Endowment Fund P60DK020541

National Institute of Standards and Technology

T.C. stanbul Kltr niversitesi

National Institute of General Medical Sciences PO1 GM095467

Genome Canada

Foundation for the National Institutes of Health 5P01CA120964

Foundation for the National Institutes of Health 5P30CA006516

National Institutes of Health R01 GM20501-41

National Institutes of Health P30 DK064391

National Institutes of Health U54 GM069338

Kansas State University

National Center for Research Resources S10RR027926

National Center for Research Resources U24 DK097154

National Center for Research Resources P20 HL113452, CFI 12156

School of Medicine

Jewish General Hospital

Canadian Institutes of Health Research FDN143309

National Center for Advancing Translational Sciences UL1 TR000040

Available from: 2017-12-07 Created: 2017-12-07 Last updated: 2018-01-30Bibliographically approved

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Hyötyläinen, TuuliaOresic, Matej

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