Harmonizing Lipidomics: NIST Interlaboratory Comparison Exercise for Lipidomics using Standard Reference Material 1950 Metabolites in Frozen Human Plasma
Number of Authors: 942017 (English)In: Journal of Lipid Research, ISSN 0022-2275, E-ISSN 1539-7262, Vol. 58, no 12, p. 2275-2288Article in journal (Refereed) Published
Abstract [en]
As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950 Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each lab using a different lipidomics workflow. A total of 1527 unique lipids were measured across all laboratories, and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra- and inter-laboratory quality control and method validation. These analyses were performed using non-standardized laboratory-independent workflows. The consensus locations were also compared to a previous examination of SRM 1950 by the LIPID MAPS consortium. While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.
Place, publisher, year, edition, pages
American Society for Biochemistry and Molecular Biology, 2017. Vol. 58, no 12, p. 2275-2288
Keywords [en]
Fatty Acyls, Glycerolipids, Lipidomics, Lipids, Phospholipids, Sphingolipids, Standard Reference Material 1950, Sterols, interlaboratory comparison exercise, quantitation
National Category
Clinical Laboratory Medicine Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-62436DOI: 10.1194/jlr.M079012ISI: 000416963900004PubMedID: 28986437Scopus ID: 2-s2.0-85037028318OAI: oai:DiVA.org:oru-62436DiVA, id: diva2:1163430
Funder
Swedish Research Council, 2015-4870Swedish Heart Lung Foundation, HLF 20140469 HLF 20150640
Note
Funding Agencies:
University of Victoria U24 DK097209, UL1 TL001873
Kyushu University
Life Sciences Institute
Austrian Science Fund P26148-N19
National Research Foundation Singapore NRFI2015-05
Genome British Columbia 7203
Genome British Columbia MC3T
Genome British Columbia P01 HL034300
Genome British Columbia Spectrometry Core
Genome British Columbia 205MET
Genome British Columbia 215MET
McGill University DBI-1228622 P20-RR16475 MCB-0920663 MCB-413036 DBI-0521587 EPS-0236913
Natural Sciences and Engineering Research Council of Canada
Diabetes Research and Training Center
Japan Science and Technology Agency JPMJCR1395
McGill University
National University of Singapore
Advanced Low Carbon Technology Research and Development Program
Albert Einstein College of Medicine, Yeshiva University
Leading Edge Endowment Fund P60DK020541
National Institute of Standards and Technology
T.C. stanbul Kltr niversitesi
National Institute of General Medical Sciences PO1 GM095467
Genome Canada
Foundation for the National Institutes of Health 5P01CA120964
Foundation for the National Institutes of Health 5P30CA006516
National Institutes of Health R01 GM20501-41
National Institutes of Health P30 DK064391
National Institutes of Health U54 GM069338
Kansas State University
National Center for Research Resources S10RR027926
National Center for Research Resources U24 DK097154
National Center for Research Resources P20 HL113452, CFI 12156
School of Medicine
Jewish General Hospital
Canadian Institutes of Health Research FDN143309
National Center for Advancing Translational Sciences UL1 TR000040
2017-12-072017-12-072019-03-04Bibliographically approved