Comparisons between commercial salivary testosterone enzyme-linked immunosorbent assay kits
2017 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 77, no 8, p. 582-586Article in journal (Refereed) Published
Abstract [en]
INTRODUCTION: Measuring testosterone concentrations is of interest both in clinical situations and for research, the latter expanding rapidly during recent years. An increased demand for convenient methods has prompted a number of companies to develop enzyme-linked immunosorbent assay (ELISA) kits to measure testosterone concentrations in saliva. However, the inter-comparability of kits from different manufacturers have yet to be determined.
AIM OF STUDY: The aim of this study was to compare commercially available ELISA kits from four different manufacturers (Salimetrics, IBL, DRG and Demeditec).
METHODS: Saliva was collected from 50 participants (25 men and 25 women). Each sample was analysed by the four ELISA kits.
RESULTS: The correlations between the ELISA kits from Demeditec, DRG and Salimetrics were moderate to high with r-values > .77; however, proportional errors between the methods calls for caution. The ELISA kit from IBL malfunctioned and no results from this kit was obtained.
CONCLUSIONS: Results from studies using the ELISA kits from Demeditec, DRG and Salimetrics are generally comparable; however, translation using the formulae presented in the current study could increase the accuracy of these comparisons.
Place, publisher, year, edition, pages
Taylor & Francis, 2017. Vol. 77, no 8, p. 582-586
Keywords [en]
Androgens, immunoassay, methods, saliva, testosterone
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:oru:diva-63057DOI: 10.1080/00365513.2017.1339231ISI: 000416756100004PubMedID: 28644096Scopus ID: 2-s2.0-85021199706OAI: oai:DiVA.org:oru-63057DiVA, id: diva2:1163654
Funder
Swedish Research Council, SRC 2015-4870
Note
Funding Agencies:
Linköping University
Östergötland County
Örebro University
Region Örebro län
Forskningskommitten
2017-12-072017-12-072018-08-13Bibliographically approved