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Comparisons between commercial salivary testosterone enzyme-linked immunosorbent assay kits
Department of Neurology, Örebro University Hospital, Örebro, Sweden.
Department of Chemistry (BMC), Analytical Chemistry and Neurochemistry, Uppsala University, Uppsala, Sweden.
Departments of Clinical Chemistry and Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
Örebro University, School of Medical Sciences. Department of Neurology, Faculty of Health and Medical Sciences, Örebro University, Örebro, Sweden; Department of Clinical Chemistry and Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.ORCID iD: 0000-0002-6681-0546
2017 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 77, no 8, p. 582-586Article in journal (Refereed) Published
Abstract [en]

INTRODUCTION: Measuring testosterone concentrations is of interest both in clinical situations and for research, the latter expanding rapidly during recent years. An increased demand for convenient methods has prompted a number of companies to develop enzyme-linked immunosorbent assay (ELISA) kits to measure testosterone concentrations in saliva. However, the inter-comparability of kits from different manufacturers have yet to be determined.

AIM OF STUDY: The aim of this study was to compare commercially available ELISA kits from four different manufacturers (Salimetrics, IBL, DRG and Demeditec).

METHODS: Saliva was collected from 50 participants (25 men and 25 women). Each sample was analysed by the four ELISA kits.

RESULTS: The correlations between the ELISA kits from Demeditec, DRG and Salimetrics were moderate to high with r-values > .77; however, proportional errors between the methods calls for caution. The ELISA kit from IBL malfunctioned and no results from this kit was obtained.

CONCLUSIONS: Results from studies using the ELISA kits from Demeditec, DRG and Salimetrics are generally comparable; however, translation using the formulae presented in the current study could increase the accuracy of these comparisons.

Place, publisher, year, edition, pages
Taylor & Francis, 2017. Vol. 77, no 8, p. 582-586
Keywords [en]
Androgens, immunoassay, methods, saliva, testosterone
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:oru:diva-63057DOI: 10.1080/00365513.2017.1339231ISI: 000416756100004PubMedID: 28644096Scopus ID: 2-s2.0-85021199706OAI: oai:DiVA.org:oru-63057DiVA, id: diva2:1163654
Funder
Swedish Research Council, SRC 2015-4870
Note

Funding Agencies:

Linköping University  

Östergötland County  

Örebro University  

Region Örebro län  

Forskningskommitten 

Available from: 2017-12-07 Created: 2017-12-07 Last updated: 2018-08-13Bibliographically approved

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Ström, Jakob O.

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