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Whole genome sequencing of the emerging invasive Neisseria meningitidis serogroup W in Sweden
Örebro University, School of Medical Sciences. Örebro University Hospital. National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine.
Örebro University, School of Medical Sciences. National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine.
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden.
Örebro University, School of Medical Sciences. Örebro University Hospital. National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine.
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2017 (English)In: 14th Congress of the EMGM, European Meningococcal and Haemophilus Disease Society: Book of Abstracts, Prague: EMGM , 2017, p. 7-8Conference paper, Poster (with or without abstract) (Other academic)
Abstract [en]

Introduction: The incidence of Neisseria meningitidisserogroup W (MenW) causing invasive meningococcal disease has historically been low. In 2015 an increase in MenW was observed in Sweden when an incidence of 0.1/100,000 population (10 cases) was reported, compared to an incidence of 0.02 (2 cases), in 2014. In 2016 the number of cases had almost doubled (18 cases, incidence of 0.2). England and Wales have also reported an increase of MenW from 2009 which was determined to be due to a sublineage in the South American/UK strain, called novel UK-2013 strain1. Both the South American/UK strain cluster and the novel UK-2013 strain belong to clonal complex (cc) 11, which consists of different strains from different serogroups associated with outbreaks that have occurred around the world2.

Aim: The aim was to determine the population structure of MenW in Sweden compared to historical and international cases.

Material and methods: All invasive MenW isolates collected in Sweden between 1995 and 2016 (n=71) were whole genome sequenced on the MiSeq (Illumina) using Nextera XT library preparation kit (Illumina) and MiSeq reagent Kit v3, 600 cycles. Reads were de novo assembled using Velvet within SeqSphere (Ridom GmbH). Genomes were uploaded to the Neisseria PubMLST database and genome comparison was performed with the genome comparator tool within pubMLST, comparing 1605 species specific core genes. The generated distance matrices were visualized using SplitsTree4 V4.

Results: The most common fine type among the Swedish isolates was P1.5-2: F1-1: ST-11 (cc11) (n=31). Theisolates belonged to four different clonal complexes: cc11, cc22, cc60 and cc174, and the majority of isolates (39/71) belonged to cc11. No particular clonal complex dominated during the investigated time period except for cc11 since 2014. Core genome comparison showed that the majority of Swedish MenW isolates clustered with the South American/UK strain (n=26), six isolates clustered with the Hajj-associated strain and seven isolates were not associated to any strain. The majority of Swedish isolates in the South American/UK strain cluster, were from 2015 to 2016 and more specifically belonged to the UK sublineages: 23 isolates in the novel UK-2013 strain and three isolates in the original UK-strain.

Conclusion: In conclusion, the increase of MenW in Sweden is comprised of isolates belonging to the South American/UK sublineage, more specifically the novel UK-2013 strain currently increasing in England and Wales.

References:1 Lucidarme J, Scott KJ, Ure R, Smith A, Lindsay D, Stenmark B, et al. An international invasive meningococcal disease outbreak due to a novel and rapidly expanding serogroup W strain, Scotland and Sweden, July to August 2015. Euro Surveill. 2016;21(45):pii=303952 Lucidarme J, Hill DM, Bratcher HB, Gray SJ, du Plessis M, Tsang RS, et al. Genomic resolution of an aggressive, widespread, diverse and expanding meningococcal serogroup B, C and W lineage. The Journal of infection. 2015;71(5):544-52

Place, publisher, year, edition, pages
Prague: EMGM , 2017. p. 7-8
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:oru:diva-63317ISBN: 978-80-906662-3-8 (print)OAI: oai:DiVA.org:oru-63317DiVA, id: diva2:1165050
Conference
14th Congress of the EMGM, European Meningococcal and Haemophilus Disease Society (EMGM 2017), Prague, Czech Republic, September 18-21, 2017
Available from: 2017-12-12 Created: 2017-12-12 Last updated: 2017-12-12Bibliographically approved

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Stenmark, BiancaEriksson, LorraineJacobsson, Susanne

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