Comparison of lipid and fatty acid composition of the liver, subcutaneous and intra-abdominal adipose tissue, and serumShow others and affiliations
2010 (English)In: Obesity, ISSN 1930-7381, E-ISSN 1930-739X, Vol. 18, no 5, p. 937-944Article in journal (Refereed) Published
Abstract [en]
Ceramides may mediate saturated fat-induced insulin resistance, but there are no data comparing ceramide concentrations between human tissues. We therefore performed lipidomic analysis of human subcutaneous (SCfat) and intra-abdominal (IAfat) adipose tissue, the liver, and serum in eight subjects. The liver contained (nmol/mg tissue) significantly more ceramides (1.5-3-fold), sphingomyelins (7-8-fold), phosphatidylethanolamines (10-11-fold), lysophosphatidylcholines (7-12-fold), less ether-linked phosphatidylcholines (2-2.5-fold) but similar amounts of diacylglycerols as compared to SCfat and IAfat. The amounts of ceramides and their synthetic precursors, such as palmitic (16:0) free fatty acids and sphingomyelins, differed considerably between the tissues. The liver contained proportionally more palmitic, stearic (18:0), and long polyunsaturated fatty acids than adipose tissues. Stearoyl-CoA desaturase 1 (SCD1) activity reflected by serum, estimated from the 16:1/16:0-ratio, was closely related to that in the liver (r = 0.86, P = 0.024) but not adipose tissues. This was also true for estimated elongase (18:1/16:1, r = 0.89, P = 0.01), and Delta5 (20:4/20:3, r = 0.89, P = 0.012) and Delta6 (18:3[n-6]/18:2, r = 1.0, P < 0.001) desaturase activities. We conclude that the human liver contains higher concentrations of ceramides and saturated free fatty acids than either SCfat or IAfat.
Place, publisher, year, edition, pages
Wiley-Blackwell Publishing Inc., 2010. Vol. 18, no 5, p. 937-944
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:oru:diva-63623DOI: 10.1038/oby.2009.326ISI: 000277234800015PubMedID: 19798063Scopus ID: 2-s2.0-77951665499OAI: oai:DiVA.org:oru-63623DiVA, id: diva2:1169189
Note
Funding agencies:
Academy of Finland
Sigrid Juselius Foundation
Finnish Diabetes Research Foundation
Biomedicum Helsinki Foundation
Novo Nordisk Foundation
European Commission
LSHM-CT-2005-018734
2017-12-222017-12-222018-05-02Bibliographically approved