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Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4-induced Th2 differentiation
Turku Centre for Biotechnology, University of Turku, Turku, Finland; Åbo Akademi University, Turku, Finland.
Turku Centre for Biotechnology, University of Turku, Turku, Finland; Åbo Akademi University, Turku, Finland; National Graduate School in Computational Biology, Bioinformatics, and Biometry, Helsinki, Finland.
Turku Centre for Biotechnology, University of Turku, Turku, Finland; Åbo Akademi University, Turku, Finland; National Graduate School in Computational Biology, Bioinformatics, and Biometry, Helsinki, Finland; Department of Mathematics, University of Turku, Turku, Finland.
Turku Centre for Biotechnology, University of Turku, Turku, Finland; Åbo Akademi University, Turku, Finland; National Graduate School in Informational and Structural Biology, Turku, Finland.
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2010 (English)In: Molecular & Cellular Proteomics, ISSN 1535-9476, E-ISSN 1535-9484, Vol. 9, no 9, p. 1937-1953Article in journal (Refereed) Published
Abstract [en]

We used stable isotope labeling with 4-plex iTRAQ (isobaric tags for relative and absolute quantification) reagents and LC-MS/MS to investigate proteomic changes in the nucleus of activated human CD4(+) cells during the early stages of Th2 cell differentiation. The effects of IL-4 stimulation upon activated naïve CD4(+) cells were measured in the nuclear fractions from 6 and 24 h in three biological replicates, each using pooled cord blood samples derived from seven or more individuals. In these analyses, in the order of 800 proteins were detected with two or more peptides and quantified in three biological replicates. In addition to consistent differences observed with the nuclear localization/expression of established human Th2 and Th1 markers, there were changes that suggested the involvement of several proteins either only recently reported or otherwise not known in this context. These included SATB1 and among the novel changes detected and validated an IL-4-induced increase in the level of YB1. This unique data set from human cord blood CD4(+) T cells details an extensive list of protein determinations that compares with and complements previous data determined from the Jurkat cell nucleus.

Place, publisher, year, edition, pages
American Society for Biochemistry and Molecular Biology, 2010. Vol. 9, no 9, p. 1937-1953
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-63631DOI: 10.1074/mcp.M900483-MCP200ISI: 000281562100011PubMedID: 20467038Scopus ID: 2-s2.0-77956543007OAI: oai:DiVA.org:oru-63631DiVA, id: diva2:1169196
Funder
EU, FP7, Seventh Framework Programme, EC-FP7-SYBILLA-201106 EC-FP7-NANOMMUNE-214281 EC-FP7-DIABIMMUNE-202063
Note

Funding agencies:

National Technology Agency of Finland  

Academy of Finland 8209083 

Sigrid Juselius Foundation  

Turku University medical faculty  

National Graduate School in Computational Biology, Bioinformatics, and Biometry 

Available from: 2017-12-22 Created: 2017-12-22 Last updated: 2018-05-02Bibliographically approved

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