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Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies of body fluids and tissues
VTT Technical Research Centre of FinlandEspooFinland.
VTT Technical Research Centre of FinlandEspooFinland.
VTT Technical Research Centre of FinlandEspooFinland.
VTT Technical Research Centre of FinlandEspooFinland.
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2011 (English)In: Methods in Molecular Biology, ISSN 1064-3745, E-ISSN 1940-6029, Vol. 708, 247-257 p.Article in journal (Refereed) Published
Abstract [en]

In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS(n) mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.

Place, publisher, year, edition, pages
Humana Press, 2011. Vol. 708, 247-257 p.
National Category
Analytical Chemistry Medical Laboratory and Measurements Technologies
Identifiers
URN: urn:nbn:se:oru:diva-63639DOI: 10.1007/978-1-61737-985-7_15PubMedID: 21207295Scopus ID: 2-s2.0-79953754873OAI: oai:DiVA.org:oru-63639DiVA: diva2:1169205
Funder
EU, FP7, Seventh Framework Programme, FP7-KBBE-222639
Available from: 2017-12-22 Created: 2017-12-22 Last updated: 2018-01-23Bibliographically approved

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Oresic, Matej

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