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Human tear fluid lipidome: from composition to function
Department of Ophthalmology, Helsinki Eye Lab, University of Helsinki, Helsinki, Finland.
VTT Technical Research Centre of Finland, Espoo, Finland.
Örebro University, School of Medical Sciences. VTT Technical Research Centre of Finland, Espoo, Finland.ORCID iD: 0000-0002-2856-9165
National Institute for Health and Welfare and FIMM Institute for Molecular Medicine Finland, Helsinki, Finland.
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2011 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 6, no 5, e19553Article in journal (Refereed) Published
Abstract [en]

We have explored human aqueous tear fluid lipidome with an emphasis to identify the major lipids. We also address the physiological significance of the lipidome. The tears were analysed using thin layer chromatographic, enzymatic and mass spectrometric techniques. To emphasize the physiological aspect of the lipidome, we modelled the spreading of the non-polar tear fluid lipids at air-water interface in macroscopic scale with olive oil and egg yolk phosphatidylcholine. Based on enzymatic analysis the respective concentrations of choline-containing lipids, triglycerides, and cholesteryl esters were 48±14, 10±0, and 21±18 µM. Ultra performance liquid chromatography quadrupole time of flight mass spectrometry analysis showed that phosphatidylcholine and phosphatidylethanolamine were the two most common polar lipids comprising 88±6% of all identified lipids. Triglycerides were the only non-polar lipids detected in mass spectrometric analysis i.e. no cholesteryl or wax esters were identified. The spreading experiments show that the presence of polar lipids is an absolute necessity for a proper spreading of non-polar tear fluid lipids. We provide evidence that polar lipids are the most common lipid species. Furthermore, we provide a physiological rationale for the observed lipid composition. The results open insights into the functional role of lipids in the tear fluid and also aids in providing new means to understand and treat diseases of the ocular surface.

Place, publisher, year, edition, pages
Public Library of Science , 2011. Vol. 6, no 5, e19553
National Category
Ophthalmology
Identifiers
URN: urn:nbn:se:oru:diva-63646DOI: 10.1371/journal.pone.0019553ISI: 000290256400022PubMedID: 21573170Scopus ID: 2-s2.0-79955788145OAI: oai:DiVA.org:oru-63646DiVA: diva2:1169211
Note

Funding agencies:

Finnish Eye Foundation  

Academy of Finland  

Finnish Foundation for Cardiovascular Research  

Sigrid Juselius Foundation

Available from: 2017-12-22 Created: 2017-12-22 Last updated: 2018-01-22Bibliographically approved

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Oresic, Matej

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