oru.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
15-Hydroxyprostaglandin dehydrogenase associates with poor prognosis in breast cancer, induces epithelial-mesenchymal transition, and promotes cell migration in cultured breast cancer cells
Medical Biotechnology, VTT Technical Research Centre of Finland, Turku, Finland; Centre for Biotechnology, University of Turku, Turku, Finland.
Medical Biotechnology, VTT Technical Research Centre of Finland, Turku, Finland; Centre for Biotechnology, University of Turku, Turku, Finland.
Institute of Tumour Biology, Centre of Experimental Medicine, University Medical Centre, Hamburg-Eppendorf, Germany.
Institute of Tumour Biology, Centre of Experimental Medicine, University Medical Centre, Hamburg-Eppendorf, Germany.
Show others and affiliations
2012 (English)In: Journal of Pathology, ISSN 0022-3417, E-ISSN 1096-9896, Vol. 226, no 4, p. 674-686Article in journal (Refereed) Published
Abstract [en]

Breast cancer is the most frequent cancer and the leading cause of cancer-related deaths in women worldwide. The prognosis of breast cancer is tightly correlated with the degree of spread beyond the primary tumour. Arachidonic acid (AA) and prostaglandin E(2) (PGE(2)) are known to regulate tumour metastasis enabling epithelial-mesenchymal transition (EMT). However, the detailed role of 15-hydroxyprostaglandin dehydrogenase (HPGD), the key enzyme degrading prostaglandin E(2) , remains unclear in breast cancer. Here, we show that HPGD mRNA is overexpressed in a subset of clinical breast cancers compared to normal breast tissue samples and that high HPGD mRNA expression associates with poor prognosis. Immunohistochemical staining of primary breast cancer and lymph node metastasis tissue samples confirmed high HPGD protein expression in 20% of the samples, as well as associated HPGD expression with aggressive characteristics, such as increased risk of disease relapse and shorter disease-free survival. Results from cultured cells indicated abundant HPGD expression in highly metastatic breast cancer cells, and impairment of HPGD expression using RNA interference led to a significant decrease in transforming growth factor-β signalling, in cellular arachidonic acid levels as well as in cell migration. Furthermore, gene expression microarray analysis followed by quantitative RT-PCR validation showed that HPGD silencing decreased aryl hydrocarbon receptor signalling and induced mesenchymal-epithelial transition. In conclusion, our results indicate that HPGD is highly expressed in metastatic and aggressive breast cancer and promotes EMT and migration in breast cancer cells.

Place, publisher, year, edition, pages
John Wiley & Sons, 2012. Vol. 226, no 4, p. 674-686
National Category
Cancer and Oncology Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:oru:diva-63658DOI: 10.1002/path.3956ISI: 000299779300012PubMedID: 22072156Scopus ID: 2-s2.0-84856746129OAI: oai:DiVA.org:oru-63658DiVA, id: diva2:1169221
Note

Funding agencies:

Cancer Organizations of Finland  

Sigrid Juselius Foundation  

TIME  

Academy of Finland 

Available from: 2017-12-22 Created: 2017-12-22 Last updated: 2018-05-15Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Oresic, Matej

Search in DiVA

By author/editor
Oresic, Matej
By organisation
School of Medical Sciences
In the same journal
Journal of Pathology
Cancer and OncologyCell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 33 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf